Sun Chong, Li Jianmin, Wang Bin, Shangguan Junjie, Figini Matteo, Shang Na, Pan Liang, Zhang Zhuoli
Department of Orthopedics, Qilu Hospital, Shandong University, Jinan, Shandong, China; Department of Radiology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
Department of Orthopedics, Qilu Hospital, Shandong University, Jinan, Shandong, China.
J Immunol Methods. 2018 Jan;452:46-52. doi: 10.1016/j.jim.2017.10.006. Epub 2017 Oct 21.
To test the hypotheses that pathological biomarkers including CD34 positive endothelial cell and microvessel density (MVD) of the primary breast tumor can be used to predict the probability of occurrence for bone metastases and provide information for appropriate therapeutic strategies at an early stage.
Three groups of CD34 immunohistochemical stained slices (n=60) were acquired from surgical specimens of sixty patients including non-metastasis (group 1), only lymph node metastasis (group 2), and bone metastasis (group 3). MVD was measured by TissueGnostics system.
The MVD measurement results were 57.14±23.00 in group 1, 86.44±21.13 in group 2, and 126.85±47.89 in group 3. There were statistical differences between group 1 and group 2 (P=0.0002), between group 2 and group 3 (P=0.0014) and between group 1 and group 3 (P<0.0001). The strong correlations were found between CD34 positive cell measurement and its percentage (group 1, r=0.74, P=0.0002; group 2, r=0.62, P=0.0034; group 3, r=0.84, P<0.0001), and between CD34 positive endothelial cell measurement and MVD (r=0.61, P<0.0001).
Quantitative CD34 positive endothelial cell and MVD measurements of the primary breast tumor have a strong correlation with the occurrence rate of bone metastases, which predicts the probability of occurrence for bone metastases at an early stage.
验证以下假设，即原发性乳腺肿瘤的病理生物标志物，包括CD34阳性内皮细胞和微血管密度（MVD），可用于预测骨转移发生的可能性，并为早期采取适当的治疗策略提供信息。
从60例患者的手术标本中获取三组CD34免疫组化染色切片（n = 60），包括无转移组（第1组）、仅淋巴结转移组（第2组）和骨转移组（第3组）。采用TissueGnostics系统测量MVD。
第1组的MVD测量结果为57.14±23.00，第2组为86.44±21.13，第3组为126.85±47.89。第1组和第2组之间（P = 0.0002）、第2组和第3组之间（P = 0.0014）以及第1组和第3组之间（P < 0.0001）存在统计学差异。CD34阳性细胞测量值与其百分比之间存在强相关性（第1组，r = 0.74，P = 0.0002；第2组，r = 0.62，P = 0.0034；第3组，r = 0.84，P < 0.0001），并且CD34阳性内皮细胞测量值与MVD之间也存在强相关性（r = 0.61，P < 0.0001）。
原发性乳腺肿瘤中CD34阳性内皮细胞和MVD的定量测量与骨转移发生率密切相关，可在早期预测骨转移发生的可能性。
