Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, P.O. Box 13145-784, Tehran, Iran.
Department of Toxicology and Pharmacology, School of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran.
Inflammopharmacology. 2018 Jun;26(3):851-859. doi: 10.1007/s10787-017-0409-1. Epub 2017 Oct 24.
The aim of the present study is to investigate the protective effects of Foeniculum vulgare essential oil on intestinal inflammation through the inhibition of NF-kB pathway in acetic acid-induced rat colitis.
Acute colitis was induced by intra-rectal administration of 2 mL of diluted acetic acid (4%) solution. Two hours after the induction of colitis, 0.2% tween 80 in normal saline, dexamethasone (2 mg/kg) and F. vulgare essential oil (100, 200, 400 mg/kg) were administered to the animals by oral gavage and continued for 5 consecutive days. Assessment of macroscopic and microscopic lesions was done. MPO activity was evaluated by biochemical method. Furthermore, TNF-α activity was detected by immunohistochemistry (IHC) and the expression level of p-NF-kB p65 protein was measured by western blot analysis.
Dexamethasone and F. vulgare essential oil (200, 400 mg/kg) reduced the macroscopic and microscopic lesions compared to the acetic acid group (p < 0.01, p < 0.001). In addition, these agents decreased the activity of MPO (p < 0.01, p < 0.001) and the expression of TNF-α positive cells (p < 0.05, p < 0.01, p < 0.001) in the colon tissue compared to acetic acid group. Furthermore, they inhibited acetic acid-induced expression of p-NF-kB p65 protein (p < 0.05, p < 0.001).
It is proposed that the anti-inflammatory activity of F. vulgare essential oil on acetic acid-induced colitis in rats may involve the inhibition of NF-kB pathway.
本研究旨在通过抑制 NF-kB 通路来探讨小茴香精油对乙酸诱导的大鼠结肠炎的肠道炎症的保护作用。
通过直肠内给予 2 mL 稀释的乙酸（4%）溶液诱导急性结肠炎。在结肠炎诱导后 2 小时，通过口服灌胃给予 0.2%吐温 80 生理盐水、地塞米松（2 mg/kg）和小茴香精油（100、200、400 mg/kg），并连续 5 天给药。评估宏观和微观病变。通过生化方法评估 MPO 活性。此外，通过免疫组织化学（IHC）检测 TNF-α 活性，并通过 Western blot 分析测量 p-NF-kB p65 蛋白的表达水平。
与乙酸组相比，地塞米松和小茴香精油（200、400 mg/kg）降低了宏观和微观病变（p < 0.01，p < 0.001）。此外，这些药物降低了 MPO 活性（p < 0.01，p < 0.001）和结肠组织中 TNF-α 阳性细胞的表达（p < 0.05，p < 0.01，p < 0.001）与乙酸组相比。此外，它们抑制了乙酸诱导的 p-NF-kB p65 蛋白的表达（p < 0.05，p < 0.001）。
提出小茴香精油对乙酸诱导的大鼠结肠炎的抗炎活性可能涉及抑制 NF-kB 通路。
