Li Xiaolan, Xu Xueqin, Huang Shansong, Zhou Yun, Jia Haijiang
Technology Center, China Tobacco Guangxi Industrial Co., Ltd, Nanning 530001, China E-mail:
Water Sci Technol. 2017 Oct;76(7-8):2075-2084. doi: 10.2166/wst.2017.313.
Methane production from waste activated sludge (WAS) anaerobic digestion is always low due to slow hydrolysis rate and inappropriate ratio of carbon to nitrogen (C/N). In this work, a novel approach, i.e., co-digestion of WAS and tobacco waste (TW) using ozone pretreatment, to greatly enhance the production of methane is reported. Experimental results showed the optimal C/N and ozone dosage for methane production was 24:1 and 90 mg/g suspended solids, and the corresponding methane production was 203.6 mL/g volatile suspended solids, which was 1.3-fold that in mono-WAS digestion. Further investigation showed the co-digestion of WAS and TW was beneficial to the consumptions of protein and cellulose; also, the presence of ozone enhanced the disruption of organic substrates and production of short chain fatty acids, which provided sufficient digestion substrates for methane generation. Analysis of microbial community structure suggested that members of the phyla Bacteroidetes and Firmicutes were the dominant species when ozone pretreatment was applied. The findings obtained in this work might be of great importance for the treatment of WAS and TW.
由于水解速率缓慢以及碳氮比（C/N）不合适，废弃活性污泥（WAS）厌氧消化产生的甲烷量一直很低。在这项工作中，报道了一种新方法，即使用臭氧预处理对WAS和烟草废弃物（TW）进行共消化，以大幅提高甲烷产量。实验结果表明，甲烷生产的最佳C/N和臭氧剂量分别为24:1和90 mg/g悬浮固体，相应的甲烷产量为203.6 mL/g挥发性悬浮固体，是WAS单消化产量的1.3倍。进一步研究表明，WAS和TW的共消化有利于蛋白质和纤维素的消耗；此外，臭氧的存在增强了有机底物的分解和短链脂肪酸的产生，为甲烷生成提供了充足的消化底物。微生物群落结构分析表明，应用臭氧预处理时，拟杆菌门和厚壁菌门的成员是优势物种。这项工作中获得的研究结果可能对WAS和TW的处理具有重要意义。
