Department of Electrical and Computer Engineering, University of Minnesota , Minneapolis, Minnesota 55455, United States.
Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota , St. Paul, Minnesota 55108, United States.
ACS Sens. 2017 Nov 22;2(11):1594-1601. doi: 10.1021/acssensors.7b00432. Epub 2017 Oct 25.
Influenza A virus (IAV) is a common respiratory pathogen infecting many hosts including humans, pigs (swine influenza virus or SIV), and birds (avian influenza virus or AIV). Monitoring swine and avian influenza viruses in the wild, farms, and live poultry markets is of great significance for human and veterinary public health. A portable, sensitive, and quantitative immunoassay device will be of high demand especially in the rural and resource-limited areas. We report herein our Z-Lab point-of-care (POC) device for sensitive and specific detection of swine influenza viruses with minimum sample handling and laboratory skill requirements. In the present study, a portable and quantitative immunoassay platform based on giant magnetoresistive (GMR) technology is used for the detection of IAV nucleoprotein (NP) and purified H3N2v. Z-Lab displays quantitative results in less than 10 min with sensitivities down to 15 ng/mL and 125 TCID/mL for IAV nucleoprotein and purified H3N2v, respectively. This platform allows lab-testing to be performed outdoors and opens up the applications of immunoassays in nonclinical settings.
甲型流感病毒（IAV）是一种常见的呼吸道病原体，可感染包括人类、猪（猪流感病毒或 SIV）和鸟类（禽流感病毒或 AIV）在内的许多宿主。监测野生、农场和活禽市场中的猪流感和禽流感病毒对于人类和兽医公共卫生具有重要意义。便携式、灵敏和定量的免疫检测设备的需求将非常高，特别是在农村和资源有限的地区。我们在此报告了我们的 Z-Lab 即时检测（POC）设备，用于灵敏和特异性检测猪流感病毒，该设备对样本处理和实验室技能的要求最低。在本研究中，一种基于巨磁电阻（GMR）技术的便携式和定量免疫检测平台被用于检测甲型流感病毒核蛋白（NP）和纯化的 H3N2v。Z-Lab 在不到 10 分钟内显示定量结果，对甲型流感病毒核蛋白和纯化的 H3N2v 的灵敏度分别达到 15ng/mL 和 125TCID/mL。该平台允许在户外进行实验室检测，并开辟了免疫检测在非临床环境中的应用。
