Feng S H, Woodley-Miller C, Chao L, Wang A C
Department of Microbiology, Medical University of South Carolina, Charleston 29425-2230.
Immunol Lett. 1988 Oct;19(2):109-13. doi: 10.1016/0165-2478(88)90128-9.
We have previously shown that three human T cell lines (MOLT-3, HUT-78 and HUT-102) were able to react with anti-human brain Thy-1 sera by cell surface immunofluorescence. However, the possibility that the antisera might cross-react with molecules other than Thy-1 could not be entirely excluded. In this report, mRNA prepared from these three T cell lines as well as from a murine T cell line (EL4) and a human B cell line (Raji) was subjected to Northern blot analysis and probed with a murine Thy-1.2 gene fragment. The result confirms our cell surface immunofluorescence data and indicates that HUT-78 and HUT-102 cells have approximately 20-fold more of the Thy-1 mRNA than MOLT-3 cells do. The Thy-1 mRNA was not detectable in the human B cell line Raji. This work is the first demonstration that the Thy-1 gene is expressed in human T cell lines. The finding is helpful in clarifying the current confusion regarding the expression of Thy-1 in human lymphoid cells and it also provides a possible model system for exploring the function of Thy-1 in cultured human T lymphocytes.
我们之前已经表明，三种人类T细胞系（MOLT-3、HUT-78和HUT-102）能够通过细胞表面免疫荧光与抗人类脑Thy-1血清发生反应。然而，不能完全排除抗血清可能与Thy-1以外的分子发生交叉反应的可能性。在本报告中，从这三种T细胞系以及一种小鼠T细胞系（EL4）和一种人类B细胞系（Raji）制备的mRNA进行了Northern印迹分析，并用小鼠Thy-1.2基因片段进行探测。结果证实了我们的细胞表面免疫荧光数据，并表明HUT-78和HUT-102细胞的Thy-1 mRNA比MOLT-3细胞多约20倍。在人类B细胞系Raji中未检测到Thy-1 mRNA。这项工作首次证明Thy-1基因在人类T细胞系中表达。这一发现有助于澄清目前关于Thy-1在人类淋巴细胞中表达的困惑，也为探索Thy-1在培养的人类T淋巴细胞中的功能提供了一个可能的模型系统。
