Zhao Ran, Chen Dong, Wu Hualing
Tea Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong 510640, China; Key Laboratory of Ministry of Education for Tea Science, Hunan Agriculture University, Changsha, Hunan 410128, China; Guangdong Key Laboratory of Tea Plant Resources Innovation and Utilization, Guangzhou, Guangdong 510640, China.
Tea Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong 510640, China; Guangdong Key Laboratory of Tea Plant Resources Innovation and Utilization, Guangzhou, Guangdong 510640, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Nov 15;1068-1069:149-156. doi: 10.1016/j.jchromb.2017.10.002. Epub 2017 Oct 4.
To evaluate effects of Pu-erh ripened tea in hyperuricemic mice, a mouse hyperuricemia model was developed by oral administration of potassium oxonate for 7 d. Serum metabolomics, based on gas chromatography-mass spectrometry, was used to generate metabolic profiles from normal control, hyperuricemic and allopurinol-treated hyperuricemic mice, as well as hyperuricemic mice given Pu-erh ripened tea at three doses. Pu-erh ripened tea significantly lowered serum uric acid levels. Twelve potential biomarkers associated with hyperuricemia were identified. Pu-erh ripened tea and allopurinol differed in their metabolic effects in the hyperuricemic mice. Levels of glutamic acid, indolelactate, L-allothreonine, nicotinoylglycine, isoleucine, l-cysteine and glycocyamine, all involved in amino acid metabolism, were significantly changed in hyperuricemic mice treated Pu-erh ripened tea. Thus, modulating amino acid metabolism might be the primary mechanism of anti-hyperuricemia by Pu-erh ripened tea.
为评估普洱茶熟茶对高尿酸血症小鼠的影响，通过口服氧嗪酸钾7天建立小鼠高尿酸血症模型。基于气相色谱-质谱联用的血清代谢组学方法，用于生成正常对照、高尿酸血症及别嘌醇治疗的高尿酸血症小鼠，以及给予三种剂量普洱茶熟茶的高尿酸血症小鼠的代谢图谱。普洱茶熟茶显著降低血清尿酸水平。鉴定出12种与高尿酸血症相关的潜在生物标志物。普洱茶熟茶和别嘌醇在高尿酸血症小鼠中的代谢作用不同。在接受普洱茶熟茶治疗的高尿酸血症小鼠中，所有参与氨基酸代谢的谷氨酸、吲哚乳酸、L-别苏氨酸、烟酰甘氨酸、异亮氨酸、L-半胱氨酸和胍基乙酸水平均发生显著变化。因此，调节氨基酸代谢可能是普洱茶熟茶抗高尿酸血症的主要机制。
