Ye Jing, Dong Xiawei, Jiang Xuerui, Jiang Hui, Li Chen-Zhong, Wang Xuemei
School of Biological Science and Medical Engineering, State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China.
Nanobioengineering/Bioelectronics Lab, Department of Biomedical Engineering, Florida International University, Miami, FL 33174, USA.
Oncotarget. 2017 Jul 1;8(42):72082-72095. doi: 10.18632/oncotarget.18914. eCollection 2017 Sep 22.
Fluorescence imaging as an attractive diagnostic technique is widely employed for early diagnosis of cancer. Self-biosynthesized fluorescent Eu complex in Hela cells have realized specifically and accurately fluorescence imaging for cancer cells. But the molecular mechanism of the biosynthesized process is still unclear. In order to reveal this mechanism, we have investigated whole-genome expression profiles with cDNA microarray, incubated with Eu solution in Hela cells for 24 h. Methylthiazoltetrazolium (MTT) assay and laser confocal fluorescence microscopy study showed the low cytotoxicity and specifically fluorescence imaging of Eu complex in Hela cells. It is observed that 563 up-regulated genes and 274 down-regulated genes were differentially expressed. Meanwhile, quantitative RT-PCR was utilized to measure the expression of some important genes, which validated the results of microarray data analysis. Besides, GO analysis showed that a wide range of differential expression functional genes involved in three groups, including cellular component, molecular function and cellular biological process. It was evident that some important biological pathways were apparently affected through KEGG pathway analysis, including focal adhesion pathway and PI3K (phosphatidylinositol 3' -kinase)-Akt signaling pathway, which can influence glycolytic metabolism and NAD(P)H-oxidases metabolic pathway.
荧光成像作为一种有吸引力的诊断技术，被广泛应用于癌症的早期诊断。在Hela细胞中自生物合成的荧光铕复合物已实现对癌细胞的特异性和准确的荧光成像。但是生物合成过程的分子机制仍不清楚。为了揭示这一机制，我们用cDNA微阵列研究了全基因组表达谱，将Hela细胞与铕溶液孵育24小时。甲基噻唑四唑（MTT）测定和激光共聚焦荧光显微镜研究表明铕复合物在Hela细胞中具有低细胞毒性和特异性荧光成像。观察到563个上调基因和274个下调基因差异表达。同时，利用定量RT-PCR测量一些重要基因的表达，验证了微阵列数据分析的结果。此外，基因本体（GO）分析表明，广泛的差异表达功能基因涉及细胞组分、分子功能和细胞生物学过程三组。显然，通过京都基因与基因组百科全书（KEGG）通路分析，一些重要的生物学途径受到明显影响，包括粘着斑通路和磷脂酰肌醇3'-激酶（PI3K）-Akt信号通路，这两条通路可影响糖酵解代谢和NAD（P）H氧化酶代谢途径。
