Chiang G L, Cheong W H, Samawickrema W A, Eng K L
Institute for Medical Research, Kuala Lumpur, Malaysia.
J Am Mosq Control Assoc. 1986 Dec;2(4):545-7.
Methods are described for the laboratory colonization of Coquillettidia crassipes. The highest rate of insemination occurred in 60 x 60 x 120 cm cages and better insemination in laboratory adapted F15 generation. Embryonation and hatchability of eggs ranged from 69.6 to 97.9% and 63.3 to 94.3% respectively. Gravid females laid egg rafts on water in 500 ml breakers with small leaves of Salvinia for resting. Newly hatched larvae were set up in a basal medium of guinea pig dung and water or liver powder, yeast powder and water. Larvae attached to aquatic plants or 'Keaykolour' ruffia snow white paper. The cultures with paper gave better yields. At present 21 generations of Cq. crassipes have been reared in the laboratory.
描述了致倦库蚊实验室定殖的方法。授精率最高出现在60×60×120厘米的笼子中，且在实验室适应的F15代中授精情况更好。卵的胚胎发育和孵化率分别为69.6%至97.9%和63.3%至94.3%。怀有身孕的雌蚊在装有槐叶萍小叶的500毫升烧杯中的水面上产下卵筏用于栖息。刚孵化出的幼虫被放置在豚鼠粪便与水的基础培养基中，或肝粉、酵母粉与水的基础培养基中。幼虫附着在水生植物或“Keaykolour”拉菲雪白纸上。有纸的培养物产量更高。目前已在实验室中饲养了21代致倦库蚊。
