Soares-da-Silva P, Caramona M M
Laboratorio de Farmacologia, Faculdade de Medicina, Porto, Portugal.
J Pharm Pharmacol. 1988 Aug;40(8):534-8. doi: 10.1111/j.2042-7158.1988.tb05297.x.
Methylene blue (3, 10 and 30 microM) increased the spontaneous outflow of endogenous dopamine and noradrenaline from sympathetic nerves supplying the dog mesenteric artery and drastically reduced the formation of endogenous dihydroxyphenylglycol (DOPEG). In addition, it decreased the accumulation of [3H]noradrenaline in the tissue, reduced the formation of [3H]DOPEG and [3H]normetanephrine, without altering the formation of [3H]dihydroxymandelic acid. In tissue homogenates of the same blood vessel, methylene blue 30 and 100 microM produced a significant reduction in the deamination of 5-hydroxytryptamine (5-HT), beta-phenylethylamine (beta-PEA) and tyramine. Methylene blue increased the accumulation of [3H]isoprenaline in the tissue, and markedly reduced the formation of [3H]O-methylisoprenaline ([3H]OMI). These results show that methylene blue alters the storage and disposition of the adrenergic transmitter.
亚甲蓝（3、10和30微摩尔）增加了供应犬肠系膜动脉的交感神经中内源性多巴胺和去甲肾上腺素的自发释放，并显著减少了内源性二羟基苯乙二醇（DOPEG）的形成。此外，它减少了组织中[3H]去甲肾上腺素的积累，降低了[3H]DOPEG和[3H]去甲变肾上腺素的形成，而不改变[3H]二羟基扁桃酸的形成。在同一血管的组织匀浆中，30和100微摩尔的亚甲蓝显著降低了5-羟色胺（5-HT）、β-苯乙胺（β-PEA）和酪胺的脱氨基作用。亚甲蓝增加了组织中[3H]异丙肾上腺素的积累，并显著降低了[3H]O-甲基异丙肾上腺素（[3H]OMI）的形成。这些结果表明亚甲蓝改变了肾上腺素能递质的储存和处置。
