Que Wen-Zhong, Chen Jun-Min
Department of Rheumatology, The Affiliated Nanping First Hospital of Fujian Medical University, Nanping 353000, Fujian Province, China.
Department of Hematology & Rheumatology, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, Fujian Province, China. E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2017 Oct;25(5):1426-1430. doi: 10.7534/j.issn.1009-2137.2017.05.025.
To investigate if NS-398 could enhance the chemosensitivity of Bortezomib (BOR) on human multiple myeloma RPMI 8226 cells.
After the treatment of NS-398 combined with BOR, MTT assay was used to detect the proliferation inhibition effect on human multiply myeloma RPMI 8226 cells in vitro, Flow cytometry was used to analyze their effect of apoptosis and cell cycle; the caspase-3 activity of different treatment group was detected by using ELISA and the activity of Cox-2 was measured by using Cox-2 activity assay kit.
The inhibitory rate of NS-398 combined with BOR was higher than that of NS-398 or BOR alone(Q>0.85). After treatment of NS-398 combined with BOR, the percentage of cells arrested in the G/G phase and apoptotic rate were both higher than that of treatment with each drug alone(Q>1.15). The caspase-3 activity in cells treated with combined of NS-398 and BOR was significantly higher than that of treatment of each drug alone(Q>1.15).
NS-398 combined with BOR shows a synergistic effect on the growth inhibition of RPMI 8226 cells in vitro.
研究NS-398是否能增强硼替佐米（BOR）对人多发性骨髓瘤RPMI 8226细胞的化疗敏感性。
用NS-398联合BOR处理后，采用MTT法检测其对人多发性骨髓瘤RPMI 8226细胞的体外增殖抑制作用，用流式细胞术分析其凋亡及细胞周期效应；采用ELISA检测不同处理组的半胱天冬酶-3活性，用Cox-2活性检测试剂盒检测Cox-2活性。
NS-398联合BOR的抑制率高于单独使用NS-398或BOR（Q>0.85）。NS-398联合BOR处理后，G/G期阻滞细胞百分比和凋亡率均高于单独使用每种药物处理（Q>1.15）。NS-398与BOR联合处理的细胞中半胱天冬酶-3活性显著高于单独使用每种药物处理（Q>1.15）。
NS-398联合BOR对体外RPMI 8226细胞的生长抑制具有协同作用。
