Chanal J L, Audran M, Sicard M T, Briley M
Faculty of Pharmacy, University of Montpellier, France.
J Pharm Pharmacol. 1988 Aug;40(8):584-5. doi: 10.1111/j.2042-7158.1988.tb05311.x.
The absorption and brain penetration of [3H]pyroglutamate was determined after oral administration to rats. Gas-liquid chromatography of the methylated derivatives followed by mass fragmentometry was used to analyse the plasma and brain levels of pyroglutamate. [3H]Pyroglutamate was separated from other labelled compounds by thin layer chromatography. The administration of 500 mg kg-1 [3H]pyroglutamate resulted in a 30-fold increase in plasma levels and a doubling in the brain levels. Over 60% of the cerebral radioactivity was present as [3H]pyroglutamate demonstrating that pyroglutamate is not only well absorbed but also penetrates in significant amounts into the brain.
给大鼠口服[³H]焦谷氨酸后，测定其吸收和脑渗透情况。采用甲基化衍生物的气-液色谱法，随后进行质谱分析，以分析焦谷氨酸的血浆和脑内水平。通过薄层色谱法将[³H]焦谷氨酸与其他标记化合物分离。给予500mg/kg[³H]焦谷氨酸后，血浆水平增加了30倍，脑内水平增加了一倍。超过60%的脑放射性以[³H]焦谷氨酸形式存在，这表明焦谷氨酸不仅吸收良好，而且大量渗透进入脑内。
