Ickrath Pascal, Kleinsasser Norbert, Ding Xin, Ginzkey Christian, Beyersdorf Niklas, Hagen Rudolf, Kerkau Thomas, Hackenberg Stephan
From the Department of Otorhinolaryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, University of Wuerzburg, Würzburg, Germany.
Institute for Virology and Immunobiology, University of Wuerzburg, Würzburg, Germany.
Allergy Rhinol (Providence). 2017 Oct 1;8(3):139-147. doi: 10.2500/ar.2017.8.0214.
There is an ongoing discussion concerning the potential origins of chronic rhinosinusitis with nasal polyposis (CRSwNP).
The aim of this study was to quantify subpopulations of T cells in peripheral blood and nasal polyps in CRSwNP to examine their influence on the etiology of this disease.
Tissue and blood samples were collected from 11 patients who underwent nasal sinus surgery, and these samples were analyzed by multicolor flow cytometry.
There was a significantly lower frequency of CD4+ T-helper (Th) cells and a significantly higher frequency of CD8+ T cells among lymphocytes isolated from nasal polyps compared with peripheral blood mononuclear cells (PBMC). In both T-cell subpopulations, a shift mainly from naive T cells among peripheral blood lymphocytes toward an effector memory and terminally differentiated subtype predominance in nasal polyps was observed. Among CD4+ T cells, the frequencies of cluster of differentiation (CD) 45RA- Forkhead-Box-Protein P3high (FoxP3high) cytotoxic T-lymphocyte-associated Protein 4high (CTLA-4high) activated regulatory T (Treg) cells, and CD45RA- Forkhead-Box-Protein P3low (FoxP3low) memory T cells were significantly increased in nasal polyps compared with PBMC.
In this study, we presented a detailed characterization of CD4+ and CD8+ T-cell subpopulations in patients with CRSwNP. CD8+ T cells were more prominent in nasal polyps than in CD4+ T cells. Both nasal CD8+ T cells and CD4+ T cells predominantly had an effector memory phenotype. Among CD4+ T cells, activated Treg cells were increased in nasal polyps compared with PBMC. The data point toward a local regulation of T-cell composition within the microenvironment of nasal polyps, which might be further exploited in the future to develop novel immunotherapeutic strategies.
关于伴鼻息肉的慢性鼻窦炎（CRSwNP）的潜在起源存在持续的讨论。
本研究的目的是量化CRSwNP患者外周血和鼻息肉中T细胞亚群，以研究它们对这种疾病病因的影响。
收集11例接受鼻窦手术患者的组织和血液样本，并通过多色流式细胞术对这些样本进行分析。
与外周血单个核细胞（PBMC）相比，从鼻息肉分离的淋巴细胞中CD4 +辅助性T（Th）细胞频率显著降低，CD8 + T细胞频率显著升高。在两个T细胞亚群中，均观察到主要从外周血淋巴细胞中的初始T细胞向鼻息肉中效应记忆和终末分化亚型占优势的转变。在CD4 + T细胞中，与PBMC相比，鼻息肉中分化簇（CD）45RA - 叉头框蛋白P3高（FoxP3高）细胞毒性T淋巴细胞相关蛋白4高（CTLA - 4高）活化调节性T（Treg）细胞以及CD45RA - 叉头框蛋白P3低（FoxP3低）记忆T细胞的频率显著增加。
在本研究中，我们对CRSwNP患者的CD4 +和CD8 + T细胞亚群进行了详细表征。CD8 + T细胞在鼻息肉中比CD4 + T细胞更突出。鼻CD8 + T细胞和CD4 + T细胞主要都具有效应记忆表型。在CD4 + T细胞中，与PBMC相比，鼻息肉中活化Treg细胞增加。这些数据表明鼻息肉微环境中T细胞组成的局部调节，未来可能会进一步利用这一点来开发新的免疫治疗策略。
