State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China.
Department of Phytopathology, Institute of Phytomedicine, Faculty of Agricultural Sciences, University of Hohenheim, 70599 Stuttgart, Germany.
Plant Physiol. 2017 Dec;175(4):1853-1863. doi: 10.1104/pp.17.01223. Epub 2017 Oct 25.
RNA interference (RNAi) is a powerful genetic tool to accelerate research in plant biotechnology and control biotic stresses by manipulating target gene expression. However, the potential of RNAi in wheat to efficiently and durably control the devastating stripe rust fungus f. sp. () remained largely under explored so far. To address this issue, we generated transgenic wheat () lines expressing dsRNA targeting transcripts of We analyzed expression of and related genes, and resistance traits of the transgenic wheat lines. We show that is an important pathogenicity factor that regulates infection and development of A RNAi construct stably expressed in two independent transgenic wheat lines confers strong resistance to hyphal development is strongly restricted, and necrosis of plant cells in resistance responses was significantly induced. We conclude that trafficking of RNA molecules from wheat plants to may lead to a complex molecular dialogue between wheat and the rust pathogen. Moreover, we confirm the RNAi-based crop protection approaches can be used, to our knowledge, as a novel control strategy against rust pathogens in wheat.
RNA 干扰 (RNAi) 是一种强大的遗传工具，可通过操纵靶基因表达来加速植物生物技术研究和控制生物胁迫。然而，迄今为止，RNAi 在小麦中高效且持久地控制破坏性条锈菌 f. sp. () 的潜力在很大程度上仍未得到探索。为了解决这个问题，我们生成了表达 dsRNA 的转基因小麦 () 株系，该 dsRNA 靶向 转录本。我们分析了转基因小麦株系中 及其相关基因的表达和抗性特征。我们表明 是一个重要的致病性因子，它调节 的侵染和发育。在两个独立的转基因小麦株系中稳定表达的 RNAi 构建体赋予对 的强烈抗性，菌丝发育受到强烈限制，并且在抗性反应中植物细胞的坏死明显诱导。我们得出结论，来自小麦植物的 RNA 分子的运输可能导致小麦和锈病病原体之间的复杂分子对话。此外，我们证实，基于 RNAi 的作物保护方法可被用于（据我们所知）作为小麦锈病病原体的新型控制策略。
