Ludlam C, Palinski W, Görög P, Born G V
Department of Pharmacology, King's College, London, U.K.
Proc R Soc Lond B Biol Sci. 1988 Nov 22;235(1279):139-44. doi: 10.1098/rspb.1988.0068.
Male Wistar rats were heparinized and killed with pentobarbital. The upper and lower ends of the aortae were cannulated and the blood was washed out with saline until the washings contained calcium and sialic-acid-reacting material at minimal concentrations. The aortae were perfused with neuraminidase for 15 min. This caused the appearance of calcium as well as of sialic acids in the perfusate in total amounts of about 5.3 nmol and about 3.6 nmol per aorta respectively. The molar ratio of about 1.5 is sufficiently close to that determined for the association of calcium with sialic acids in vitro to suggest that their association is similar in vivo.
雄性Wistar大鼠经肝素化处理后，用戊巴比妥处死。将主动脉的上下两端插管，并用生理盐水冲洗血液，直至冲洗液中钙和唾液酸反应物质的浓度降至最低。用神经氨酸酶灌注主动脉15分钟。这导致灌注液中分别出现钙和唾液酸，每条主动脉的总量约为5.3纳摩尔和约3.6纳摩尔。约1.5的摩尔比与体外测定的钙与唾液酸结合的摩尔比足够接近，表明它们在体内的结合情况相似。
