Mabrouk Mokhtar M, Hammad Sherin F, El-Malla Samah F, Elshenawy Eman A
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University, Tanta, Egypt.
Luminescence. 2018 Mar;33(2):364-369. doi: 10.1002/bio.3422. Epub 2017 Oct 25.
Highly sensitive, rapid, accurate and precise synchronous fluorescence spectrofluorimetric method has been developed for simultaneous analysis of a mixture of amlodipine (AMD) and metoprolol (MET). The method relies on measuring the relative synchronous fluorescence intensity of both drugs at Δλ of 90 nm in acetate buffer solution at pH 5. The experimental parameters influencing the developed method were investigated and optimized. The method was linear over the ranges 0.2-2 μg/ml and 0.5-10 μg/ml for AMD and MET, respectively. The limits of detection were 50 ng/ml for AMD and 130 ng/ml for MET while the limits of quantitation were 150 ng/ml for AMD and 390 ng/ml for MET. The developed method was applied successfully for the determination of the two drugs in their co-formulated tablet. The mean percent recoveries were found to be 100.51 and 99.57 for AMD and MET, respectively.
已开发出高灵敏度、快速、准确且精密的同步荧光光谱荧光法，用于同时分析氨氯地平（AMD）和美托洛尔（MET）的混合物。该方法基于在pH 5的醋酸盐缓冲溶液中，在90 nm的Δλ处测量两种药物的相对同步荧光强度。对影响所开发方法的实验参数进行了研究和优化。该方法对于AMD和MET的线性范围分别为0.2 - 2 μg/ml和0.5 - 10 μg/ml。AMD的检测限为50 ng/ml，MET的检测限为130 ng/ml，而AMD的定量限为150 ng/ml，MET的定量限为390 ng/ml。所开发的方法成功应用于测定其复方片剂中的两种药物。发现AMD和MET的平均回收率分别为100.51%和99.57%。
