Long Xian-Qi, Wang Xu, Ren Xiu-Jun, Yu Xue, Tu Ya, Tang Yu-Xiu
School of Acu-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China; Department of Medicine, Qiannan Medical College for Nationalities, Duyun 558000.
School of Basic Medicine, Beijing University of Chinese Medicine, Beijing 100029.
Zhen Ci Yan Jiu. 2016 Jun 25;41(3):202-9.
To observe the influence of Electroacupuncture (EA) stimulation of different distal-proximal acupoints combination groups on pain reaction, spinal p 38 mitogen-activated protein kinase (p 38 MAPK) expression and cyclic adenosine 3', 5'-monophosphate (cAMP) content in experimental lumbago rats.
Seventy-eight male SD rats were randomized into normal, model, sham operation, EX-B 2-BL 25 (proximal acupoints, PA), BL 40-BL 60 (distal acupoints, DA) and DA+PA groups. The lumbago model was established by implanting the autologous nucleus pulposus to the site close to the left L 5 dorsal root ganglion and the surface of dura mate of spinal cord. EA (2 Hz/100 HZ,1-3 mA) was applied at bilateral EX-B 2-BL 25 or BL 40-BL 60 or both for 20 minutes, once daily for 7 days. The mechanical pain threshold was measured, and the expression of p 38 MAPK in the lumbar spinal cord was detected by immunohistochemistry and Western blot, separately. The content of cAMP in spinal cord (L 4-L 6) was measured by enzyme-linked immunosorbent assay (ELISA).
After mode-ling, the mechanical pain threshold percentages were significantly decreased on day 3, 5 and 7 (<0.01), and after EA stimulation of DA,PA and DA+PA, the decreased pain threshold was significantly increased at the 3 time-points (<0.01), and the effects of both PA and DA+PA were markedly superior to that of DA (<0.05, <0.01). Following modeling, lumbar spinal p 38 MAPK immunoactivity and protein expression, and cAMP content were obviously up-regulated (<0.01). Compared with the model group, the levels of p 38 MAPK immunoactivity and protein expression in the 3 EA groups and the sham group, and cAMP contents in both PA and DA+PA groups (not in the DA and sham groups) were considerably down-regulated (<0.01, <0.05).
EA stimulation of distal+proximal acupoints and proximal acupoints near the focus can raise pain threshold in lumbago rats, which may be associated with their effects in suppressing lumbago-induced increase of expression of spinal p 38 MAPK protein and cAMP content. The effects of EA of distal+proximal acupoints and proximal acupoints are better than those of EA of simple distal acupoints.
观察电针不同远近配穴组合组对实验性腰腿痛大鼠疼痛反应、脊髓p38丝裂原活化蛋白激酶（p38 MAPK）表达及环磷酸腺苷（cAMP）含量的影响。
将78只雄性SD大鼠随机分为正常组、模型组、假手术组、EX - B2 - BL25组（近部穴位组，PA）、BL40 - BL60组（远部穴位组，DA）和DA + PA组。通过将自体髓核植入左侧L5背根神经节附近及脊髓硬脊膜表面建立腰腿痛模型。电针（2Hz/100Hz，1 - 3mA）分别作用于双侧EX - B2 - BL25或BL40 - BL60或两组，每次20分钟，每日1次，共7天。分别测量机械性疼痛阈值，采用免疫组织化学和蛋白质印迹法检测腰段脊髓p38 MAPK的表达，采用酶联免疫吸附测定（ELISA）法检测脊髓（L4 - L6）cAMP含量。
造模后第3、5、7天机械性疼痛阈值百分比显著降低（<0.01），电针刺激DA、PA和DA + PA组后，3个时间点降低的疼痛阈值均显著升高（<0.01），且PA和DA + PA组的效果均明显优于DA组（<0.05，<0.01）。造模后，腰段脊髓p38 MAPK免疫活性、蛋白表达及cAMP含量均明显上调（<0.01）。与模型组相比，3个电针组和假手术组的p38 MAPK免疫活性和蛋白表达水平，以及PA组和DA + PA组（DA组和假手术组未出现）的cAMP含量均显著下调（<0.01，<0.05）。
电针远近配穴及病灶附近的近部穴位可提高腰腿痛大鼠的疼痛阈值，这可能与其抑制腰腿痛引起的脊髓p38 MAPK蛋白表达增加及cAMP含量升高有关。电针远近配穴及近部穴位的效果优于单纯远部穴位电针。
