Li Yu-Wei, Zhao Ji-Meng, Chen Liu, Shang Hai-Xia, Wu Lu-Yi, Bao Chun-Hui, Dou Chuan-Zi, Wu Huan-Gan, Shi Yin
Yueyang Clinical Medical College, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Shanghai Institute of Acu-moxibustion and Meridian, Shanghai 200030.
Zhen Ci Yan Jiu. 2016 Aug 25;41(4):291-7.
To compare the effects of electroacupuncture (EA) and moxibustion (Moxi) on visceral pain and expression of vanilloid receptor subtype 1 (VR 1) and heat shock protein (HSP)70 in "Tianshu" (ST 25) region in colorectal distension (CRD)-induced visceral hypersensitivity (VHS) rats.
Fifty male SD rats were randomly divided into normal control, VHS model, 43℃-moxi, 46℃-moxi, 1 mA-EA and 3 mA-EA groups (=10 in each group). The VSH model was established by CRD once daily for 14 days. EA or Moxi stimulation was applied to bilateral "Tianshu" (ST 25) for 10 min, once daily for consecutive 10 days. The abdominal withdrawal reflex (AWR) scores (0-4 points) were rated according to Al-Chaer's and coworkers' standards (2000) and the expression levels of VR 1 and HSP 70 in bilateral ST 25 area tissues detected by immunohistochemistry.
The AWR scores for 20, 40, 60 and 80 mmHg CRD pressures were significantly increased compared to the normal control group (<0.01) and notably decreased after 43℃- and 46℃-moxi, and 1 mA- and 3 mA-EA stimulation of bila-teral ST 25 in comparison with the model group (<0.05, <0.01), and the effect of 46℃-moxi was apparently superior to those of 1 mA-EA at 40 and 80 mmHg, and 3 mA-EA at 40 mmHg (<0.05). After modeling, the expression of both VR 1 and HSP 70 (percentages of area of positive-cells) in ST 25 region had no significant changes (>0.05). Compared to the model group, the expression levels of VR 1 in the 43℃-moxi and 46℃-moxi groups, and HSP 70 in the 43℃-moxi and 46℃-moxi, 1 mA-EA and 3 mA-EA groups were significantly up-regulated (<0.01), but without obvious changes in the expression of VR 1 in the 1 mA-EA and 3 mA-EA groups (>0.05). The effects of 46℃-moxi were considerably better than those of 43℃-moxi, 1 mA-EA and 3 mA-EA in up-regulating VR 1 and HSP 70 expression (<0.05, <0.01). No significant differences were found among the 43℃-moxi, 1 mA-EA and 3 mA-EA groups in the expression of VR 1 and HSP 70 (>0.05).
Moxibustion at 43℃ and 46℃ and EA at 1 mA and 3 mA, especially the 46℃-moxi, can relieve visceral pain in visceral hypersensitivity rats, which may be related to their effects in up-regulating expression of VR 1 and HSP 70 in "Tianshu" (ST 25) area.
比较电针（EA）与艾灸（Moxi）对结直肠扩张（CRD）诱导的内脏高敏（VHS）大鼠“天枢”（ST 25）区域内脏痛及香草酸受体1型（VR 1）和热休克蛋白（HSP）70表达的影响。
将50只雄性SD大鼠随机分为正常对照组、VHS模型组、43℃艾灸组、46℃艾灸组、1 mA电针组和3 mA电针组（每组10只）。通过每天1次CRD建立VSH模型，持续14天。对双侧“天枢”（ST 25）进行EA或艾灸刺激10分钟，每天1次，连续10天。根据Al-Chaer及其同事（2000年）的标准对腹部退缩反射（AWR）评分（0 - 4分），并通过免疫组织化学检测双侧ST 25区域组织中VR 1和HSP 70的表达水平。
与正常对照组相比，20、40、60和80 mmHg CRD压力下的AWR评分显著升高（P<0.01），与模型组相比，43℃和46℃艾灸、1 mA和3 mA电针刺激双侧ST 25后AWR评分显著降低（P<0.05，P<0.01），且46℃艾灸在40和80 mmHg时的效果明显优于1 mA电针，在40 mmHg时优于3 mA电针（P<0.05）。建模后，ST 25区域VR 1和HSP 70（阳性细胞面积百分比）的表达无显著变化（P>0.05）。与模型组相比，43℃和46℃艾灸组的VR 1表达水平，以及43℃和46℃艾灸组、1 mA和3 mA电针组的HSP 70表达水平均显著上调（P<0.01），但1 mA和3 mA电针组的VR 1表达无明显变化（P>0.05）。在上调VR 1和HSP 70表达方面，46℃艾灸的效果明显优于43℃艾灸、1 mA和3 mA电针（P<0.05，P<0.01）。43℃艾灸组、1 mA电针组和3 mA电针组在VR 1和HSP 70表达上无显著差异（P>0.05）。
43℃和46℃艾灸以及1 mA和3 mA电针，尤其是46℃艾灸，可缓解内脏高敏大鼠的内脏痛，这可能与其上调“天枢”（ST 25）区域VR 1和HSP 70表达的作用有关。
