Wang Chao, Xie Wen-juan, Liu Mi, Yan Jie, Zhang Jia-li, Liu Zhao, Guo Li-na
Zhen Ci Yan Jiu. 2014 Oct;39(5):372-6.
To observe the effect of electroacupuncture (EA) and moxibustion (Moxi) preconditioning of bi- lateral "Neiguan" (PC 6) on plasma endothelin (ET) and serum creatine kinase (CK) contents and myocardial hot shock protein 70 (HSP 70) expression in myocardial ischemia-reperfusion injury (MIRI) rabbits, so as to revel their mechanisms underlying prevention of myocardial ischemia.
A total of 72 New Zealand rabbits were randomly divided into sham operation, MIRI model, EA preconditioning and Moxi preconditioning groups (n = 18/group). Each group was further divided into 0 h, 24 h and 48 h (time-point) subgroups (n=6 in each subgroup). The MIRI model was established by occlusion of the anterior descending branch of the left coronary artery for 40 min and reperfusion for 60 min. The contents of plasma ET and serum CK were detected by ELISA, and myocardial HSP 70 expression was detected by immunohistochemistry. EA and Moxi preconditioning were respectively applied to bilateral PC 6 for 20 min, once daily for 5 days.
Following MIRI, contents of plasma ET and serum CK contents were significantly increased at 0 h, 24 h and 48 h in comparison with the sham group (P<0.01, P<0.05), while myo- cardial HSP 70 expression at the 3 time-points was moderately increased (P>0.05). Compared with the model groups, plasma ET contents at both 24 h and 48 h in the EA preconditioning group and at 48 h in the Moxi preconditioning group, CK contents at both 24 h and 48 h only in the EA preconditioning group were significantly down-regulated (P<0.01, P<0.05). Myocardial HSP 70 expression levels in the EA and Moxi preconditioning groups were considerably up-regulated at the three time-points in comparison with the model group(P<0.05, P<0.01).
Acupuncture and moxibustion pretreatment may suppress MIRI-induced increase of plasma ET and serum CK and up-regulate myocardial HSP 70 protein expression in MIRI rabbits, suggesting a preventive protection action on ischemic myocardium.
观察双侧“内关”（PC 6）电针（EA）和艾灸（Moxi）预处理对心肌缺血再灌注损伤（MIRI）家兔血浆内皮素（ET）、血清肌酸激酶（CK）含量及心肌热休克蛋白70（HSP 70）表达的影响，以揭示其预防心肌缺血的机制。
将72只新西兰家兔随机分为假手术组、MIRI模型组、EA预处理组和Moxi预处理组（每组18只）。每组再分为0 h、24 h和48 h（时间点）亚组（每个亚组6只）。通过结扎左冠状动脉前降支40分钟并再灌注60分钟建立MIRI模型。采用酶联免疫吸附测定法（ELISA）检测血浆ET和血清CK含量，采用免疫组织化学法检测心肌HSP 70表达。EA和Moxi预处理分别作用于双侧PC 6 20分钟，每天1次，共5天。
与假手术组相比，MIRI后0 h、24 h和48 h血浆ET和血清CK含量显著升高（P<0.01，P<0.05），而3个时间点心肌HSP 70表达中度升高（P>0.05）。与模型组相比，EA预处理组24 h和48 h以及Moxi预处理组48 h的血浆ET含量，仅EA预处理组24 h和48 h的CK含量显著下调（P<0.01，P<0.05）。与模型组相比，EA和Moxi预处理组3个时间点的心肌HSP 70表达水平均显著上调（P<0.05，P<0.01）。
针刺和艾灸预处理可能抑制MIRI诱导的MIRI家兔血浆ET和血清CK升高，并上调心肌HSP 70蛋白表达，提示对缺血心肌有预防性保护作用。
