Deng Jing-Jing, Yuan Qing, Wang Man-Xia, Yang Yi-Zong
Department of Chinese Medicine, Guangzhou 8 th People's Hospital, Guangzhou 510440, China.
College of Acu-moxibustion and Massage, Guangzhou University of Chinese Medicine, Guangzhou 510405.
Zhen Ci Yan Jiu. 2017 Aug 25;42(4):296-301.
To observe the effect of acupuncture on expression of receptor tyrosine kinase (RTK) c-kit in interstitial cells of Cajal (ICCs), carbon monoxide (CO) and heme oxygenase (HO) in colon tissue after colonic anastomosis, so as to explore the mechanism of acupuncture in improving gastrointestinal motility.
SD rats were randomly devided into control group, model group and acupuncture group. The model was established using colonic anastomosis. Each group was further devided into 3, 5, and 10 d (time-point) subgroups (=10 in each). Acupuncture was applied to acupuncture group at bilateral "Zusanli" (ST 36), "Sanyinjiao" (SP 6) and "Taichong" (LR 3) for 15 min, once daily after modeling. The first defecation time was recorded, and the intestinal propulsive rate was measured. The expression of c-kit in colon tissue was detected by immunohistochemistry. The content of CO, the activities of HO-1 and HO-2 in colon tissue were detected by biochemical method and ELISA, respectively.
Compared to the control group, the intestinal propulsive rate and the expression level of c-kit in ICCs were decreased in the model group(<0.05), the content of CO and the activity of HO-1 were increased in model 3 d and 5 d subgroups(<0.05), the activity of HO-2 was increased in model 3 d subgroup(<0.05), while the opposite results appeared in model 5 d and 10 d subgroups(<0.05). Compared to the model group, the first defecation time was shortened (<0.05), the intestinal propulsive rate and the expression level of c-kit in ICCs were increased in the acupuncture group (<0.05), the content of CO and the activity of HO-1 were decreased in acupuncture 3 d and 5 d subgroups(<0.05), the activity of HO-2 was decreased in acupuncture 3 d subgroup(<0.05), while the opposite result appeared in acupuncture 10 d subgroup(<0.05). Compared to the 3 d subgroup, the intestinal propulsive rate and the expression level of c-kit in ICCs were increased, the content of CO, the activities of HO-1 and HO-2 were decreased in both model and acupuncture 5 d subgroups (<0.05). In model 10 d subgroup, the intestinal propulsive rate and the expression level of c-kit in ICCs were increased, the content of CO and the activity of HO-1 were decreased in comparison with the model 5 d subgroup(<0.05).
Acupuncture can improve postoperative gastrointestinal motility by declining CO content and HO-1 and HO-2 activity in colon tissue, and promoting ICCs restoration.
观察针刺对结肠吻合术后结肠组织中Cajal间质细胞（ICCs）受体酪氨酸激酶（RTK）c-kit、一氧化碳（CO）及血红素氧合酶（HO）表达的影响，以探讨针刺改善胃肠动力的机制。
将SD大鼠随机分为对照组、模型组和针刺组。采用结肠吻合术建立模型。每组再分为3、5、10 d（时间点）亚组（每组n = 10）。针刺组于造模后每日针刺双侧“足三里”（ST 36）、“三阴交”（SP 6）和“太冲”（LR 3）15 min。记录首次排便时间，测量肠推进率。采用免疫组织化学法检测结肠组织中c-kit的表达。分别采用生化法和ELISA法检测结肠组织中CO含量、HO-1和HO-2的活性。
与对照组比较，模型组肠推进率及ICCs中c-kit表达水平降低（P<0.05），模型3 d和5 d亚组CO含量及HO-1活性升高（P<0.05），模型3 d亚组HO-2活性升高（P<0.05），而模型5 d和10 d亚组结果相反（P<0.05）。与模型组比较，针刺组首次排便时间缩短（P<0.05），肠推进率及ICCs中c-kit表达水平升高（P<0.05），针刺3 d和5 d亚组CO含量及HO-1活性降低（P<0.05），针刺3 d亚组HO-2活性降低（P<0.05），而针刺10 d亚组结果相反（P<0.05）。与3 d亚组比较，模型和针刺5 d亚组肠推进率及ICCs中c-kit表达水平升高，CO含量、HO-1和HO-2活性降低（P<0.05）。模型10 d亚组与模型5 d亚组比较，肠推进率及ICCs中c-kit表达水平升高，CO含量及HO-1活性降低（P<0.05）。
针刺可通过降低结肠组织中CO含量及HO-1和HO-2活性，促进ICCs恢复，从而改善术后胃肠动力。
