Zhang Cheng-Cheng, Lin Ya-Ping, Peng Yan, Yue Zeng-Hui, Chen Hai-Jiao, Yang Jian-Wen, Liu Wei-Wei, Liu Li
Graduate School, Hunan University of Chinese Medicine, Changsha 410007, China.
Key Acu-moxibustion Laboratory of Biological Information Analysis of Institute of Acupuncture, Moxibustion and Massage, Hunan University of Chinese Medicine, Changsha 410007, China.
Zhen Ci Yan Jiu. 2017 Dec 25;42(6):482-8. doi: 10.13702/j.1000-0607.2017.06.003.
To observe the effects of electroacupuncture (EA) on gastrointestinal motility and the ultrastructure of interstitial cells of Cajal (ICC) and the expressions of c-kit receptor protein and stem cell factor (SCF) mRNA in diabetic gastroparesis (DGP) rats, so as to explore its mechanism.
Fifty SD rats were randomly divided into normal, model, acupoint, non-acupoint and metoclopramide groups (n＝10 rats/group). DGP model was established by intraperitoneal injection of streptozotocin (STZ, 2%), and raised with high-sugar high-fat diet irregularly. EA (sparse-dense, 10 Hz/50 Hz, 2 mA, 20 min) was applied at "Zusanli" (ST 36), "Liangmen" (ST 21) and "Sanyinjiao" (SP 6), and the corresponding non-acupoints of the 3 acupoints, daily for 15 days. The rats in metoclopramide group received intragastric administration of metoclopramide (1.7%, 1 mL/100 g) for 15 days, once a day. Blood sugar was determined with One Touch blood glucose test paper. The gastric emptying rate (GER) and the intestinal propulsion rate (IPR) were measured by intragastric phenol red. The ultrastructure of ICC was detected by transmission electron microscopy. The expression levels of c-kit receptor protein and SCF mRNA of gastric antrum were examined respectively by Western blot and RT-PCR.
Compared with the normal group, the blood glucose significantly increased in the model group (<0.01), while the GER, IRP and the expression level of SCF mRNA in the gastric antrum significantly decreased (<0.01), and the ultrastructure of ICC appeared apoptosis-like changes. The blood glucose of the EA group was obviously decreased compared with that of the model group (<0.05); the GER and IRP significantly increased(<0.05, <0.01); the expression level of SCF mRNA increased (<0.01), the number of ICC increased and its ultrastructure was repaired. There was some relief on ICC ultrastructure in the acupoint group compared with that in the non-acupoint group; and SCF mRNA increased (<0.05). There was no significant difference on c-kit receptor expression among all the modeling groups (P＞0.05).
EA at ST 36, etc. can regulate the blood glucose and improve gastrointestinal emptying in DGP rats. The mechanism may be related to up-regulating SCF mRNA, repairing ICC ultrastructure, restoring the pacing function, and improving gastrointestinal motility.
观察电针（EA）对糖尿病胃轻瘫（DGP）大鼠胃肠动力、Cajal间质细胞（ICC）超微结构及c-kit受体蛋白和干细胞因子（SCF）mRNA表达的影响，以探讨其作用机制。
将50只SD大鼠随机分为正常组、模型组、穴位组、非穴位组和胃复安组（每组10只）。通过腹腔注射链脲佐菌素（STZ，2%）建立DGP模型，并用高糖高脂饮食不规则饲养。电针（疏密波，10Hz/50Hz，2mA，20min）分别作用于“足三里”（ST36）、“梁门”（ST21）和“三阴交”（SP6）及相应非穴位，每日1次，共15天。胃复安组大鼠灌胃给予胃复安（1.7%，1mL/100g），连续15天，每天1次。用血糖仪测定血糖。采用胃内注射酚红法测定胃排空率（GER）和肠推进率（IPR）。透射电镜观察ICC超微结构。分别采用Western blot和RT-PCR检测胃窦c-kit受体蛋白和SCF mRNA表达水平。
与正常组比较，模型组血糖显著升高（<0.01），GER、IPR及胃窦SCF mRNA表达水平显著降低（<0.01），ICC超微结构出现凋亡样改变。与模型组比较，电针组血糖明显降低（<0.05）；GER、IPR显著升高（<0.05，<0.01）；SCF mRNA表达水平升高（<0.01），ICC数量增加，超微结构修复。穴位组ICC超微结构较非穴位组有所改善；SCF mRNA升高（<0.05）。各造模组c-kit受体表达差异无统计学意义（P＞0.05）。
电针ST36等穴位可调节DGP大鼠血糖，改善胃肠排空。其机制可能与上调SCF mRNA、修复ICC超微结构、恢复起搏功能及改善胃肠动力有关。
