Laboratoire de Chimie Physique et Microbiologie pour l'Environnement (LCPME), UMR 7564 CNRS-Université de Lorraine, Villers-lès-Nancy, France.
Electrophoresis. 2018 Jan;39(2):377-385. doi: 10.1002/elps.201700352. Epub 2017 Nov 13.
SDS is commonly employed as BGE additive in CZE analysis of non-enveloped icosahedral viruses. But the way by which SDS interacts with the surface of such viruses remains to date poorly known, making complicate to understand their behavior during a run. In this article, two related bacteriophages, MS2 and Qβ, are used as model to investigate the migration mechanism of non-enveloped icosahedral viruses in SDS-based CZE. Both phages are characterized by similar size and surface charge but significantly different surface hydrophobicity (Qβ > MS2, where '>' means 'more hydrophobic than'). By comparing their electrophoretic mobility in the presence or not of SDS on both sides of the CMC, we show that surface hydrophobicity of phages is a key factor influencing their mobility and that SDS-virus association is driven by hydrophobic interactions at the surface of virions. The CZE analyses of heated MS2 particles, which over-express hydrophobic domains at their surface, confirm this finding. The correlations between the present results and others from the literature suggest that the proposed mechanism might not be exclusive to the bacteriophages examined here.
SDS 通常被用作 CZE 分析无包膜二十面体病毒的 BGE 添加物。但是，到目前为止，SDS 与这些病毒表面相互作用的方式还知之甚少，这使得在运行过程中难以理解它们的行为。在本文中，两种相关的噬菌体 MS2 和 Qβ被用作模型来研究基于 SDS 的 CZE 中非包膜二十面体病毒的迁移机制。这两种噬菌体的大小和表面电荷相似，但表面疏水性有很大差异（Qβ>MS2，其中“>”表示“比......更疏水”）。通过比较它们在 CMC 两侧存在或不存在 SDS 时的电泳迁移率，我们表明噬菌体的表面疏水性是影响其迁移率的关键因素，并且 SDS-病毒的结合是由病毒表面的疏水相互作用驱动的。对表面过度表达疏水区的加热 MS2 颗粒的 CZE 分析证实了这一发现。目前的结果与文献中的其他结果之间的相关性表明，所提出的机制可能不仅限于此处检查的噬菌体。
