He Guangjie, Liu Chenxi, Liu Xiaobo, Wang Qingzhi, Fan Aiying, Wang Songjun, Qian Xinlai
Department of Forensic Medicine, Xinxiang Medical University, Xinxiang, Henan Province, P. R. China.
Hebei Key Laboratory of Forensic Medicine, Hebei Medical University, Shijiazhuang, Hebei Province, P. R. China.
PLoS One. 2017 Oct 26;12(10):e0186994. doi: 10.1371/journal.pone.0186994. eCollection 2017.
Copper, as the third most abundant transition metal ions of human, plays an essential role in the redox reaction, signal transduction, hematopoiesis, and other physiological processes. Abnormal content of copper ions in the body will cause some diseases such as anemia, coronary heart disease, Menkes' syndrome. In this article, a new fluorescence probe L for Cu2+ was designed and synthetized by using 4-bromo-1,8 naphthalene anhydride and 2-thiophene formaldehyde as raw materials. Fluorescent probe L itself exhibited strong fluorescence, upon the addition of Cu2+ ions, the fluorescence was quenched. The fluorescent detection limit for Cu2+ ions was determined to be 1.8 μM based on a 3δ/S method. UV-vis absorption and fluorescence spectra indicated that probe L showed good selectivity and sensitivity for Cu2+, and this selectivity was not interfered by other metal ions and anions. Further cell fluorescence imaging experiments indicated that the probe L had potential to be used to examine copper ions in vivo.
铜作为人体中含量第三丰富的过渡金属离子，在氧化还原反应、信号转导、造血及其他生理过程中发挥着重要作用。体内铜离子含量异常会引发一些疾病，如贫血、冠心病、门克斯综合征。在本文中，以4-溴-1,8-萘二甲酸酐和2-噻吩甲醛为原料设计合成了一种新型的铜离子荧光探针L。荧光探针L本身呈现出较强的荧光，加入铜离子后，荧光猝灭。基于3δ/S方法测定铜离子的荧光检测限为1.8 μM。紫外-可见吸收光谱和荧光光谱表明，探针L对铜离子具有良好的选择性和灵敏度，且这种选择性不受其他金属离子和阴离子的干扰。进一步的细胞荧光成像实验表明，探针L具有用于体内检测铜离子的潜力。
