Liu Qiang, Jin Li Hua
Department of Genetics, College of Life Sciences, Northeast Forestry University, No.26 Hexing Road Xiangfang District, Harbin 150040, China.
Exp Cell Res. 2017 Dec 15;361(2):225-235. doi: 10.1016/j.yexcr.2017.10.021. Epub 2017 Oct 24.
Pluripotent stem cell activity is essential to maintain regeneration and homeostasis in the Drosophila midgut following environmental challenges. Although multiple pathways have been implicated in epithelial renewal, the underlying regulatory mechanisms and correlations between relevant genes and pathways remain elusive. In this study, we show that the zinc finger protein CG12744 plays an important role in the differentiation and regeneration of epithelial cells in response to oral infection with Erwinia carotovora carotovora 15. Knocking down CG12744 in enteroblasts decreased the post-infection proportion of enteroblasts and enterocytes and increased the post-infection number of enteroendocrine cells. In addition, in precursors, CG12744 affected the Osa, jun-N-terminal kinase and bone morphogenetic protein signaling pathways to control enterocyte differentiation. Finally, CG12744 maintained epithelial architecture and cell fate in enterocytes following an acute infectious challenge.
多能干细胞活性对于果蝇中肠在环境挑战后维持再生和内环境稳定至关重要。尽管多种途径与上皮更新有关，但相关基因和途径之间潜在的调控机制及相关性仍不清楚。在本研究中，我们发现锌指蛋白CG12744在应对胡萝卜软腐欧文氏菌15型口腔感染时，在上皮细胞的分化和再生中发挥重要作用。在成肠细胞中敲低CG12744会降低感染后成肠细胞和肠细胞的比例，并增加感染后肠内分泌细胞的数量。此外，在祖细胞中，CG12744影响Osa、Jun氨基末端激酶和骨形态发生蛋白信号通路以控制肠细胞分化。最后，在急性感染挑战后，CG12744维持肠细胞中的上皮结构和细胞命运。
