用于荧光标记生物组织光学成像的中性嵌入树脂的开发。

Development of a neutral embedding resin for optical imaging of fluorescently labeled biological tissue.

机构信息

Huazhong University of Science and Technology, Collaborative Innovation Center for Biomedical Engine, China.

Huazhong University of Science and Technology, School of Engineering Sciences, Britton Chance Center, China.

出版信息

J Biomed Opt. 2017 Oct;22(10):1-7. doi: 10.1117/1.JBO.22.10.106015.

DOI:10.1117/1.JBO.22.10.106015

PMID:29076308

Abstract

Plastic embedding is widely applied in light microscopy analyses. Previous studies have shown that embedding agents and related techniques can greatly affect the quality of biological tissue embedding and fluorescent imaging. Specifically, it is difficult to preserve endogenous fluorescence using currently available acidic commercial embedding resins and related embedding techniques directly. Here, we developed a neutral embedding resin that improved the green fluorescent protein (GFP), yellow fluorescent protein (YFP), and DsRed fluorescent intensity without adjusting the pH value of monomers or reactivating fluorescence in lye. The embedding resin had a high degree of polymerization, and its fluorescence preservation ratios for GFP, YFP, and DsRed were 126.5%, 155.8%, and 218.4%, respectively.

摘要

塑料包埋广泛应用于光学显微镜分析。先前的研究表明，包埋剂及相关技术会极大地影响生物组织的包埋和荧光成像质量。具体来说，目前常用的酸性商业包埋树脂和相关包埋技术很难直接保存内源性荧光。在这里，我们开发了一种中性包埋树脂，可以在不调节单体 pH 值或在碱液中重新激活荧光的情况下，提高绿色荧光蛋白（GFP）、黄色荧光蛋白（YFP）和 DsRed 的荧光强度。该包埋树脂的聚合度较高，其对 GFP、YFP 和 DsRed 的荧光保存率分别为 126.5%、155.8%和 218.4%。