Chen Yuhui, Cao He, Sun Dawei, Lin Changxin, Wang Liang, Huang Minjun, Jiang Huaji, Zhang Zhongmin, Jin Dadi, Zhang Baiyu, Bai Xiaochun
Department of Orthopedic, The Third Affiliated Hospital of Southern Medical University, Guangzhou 510665, China
Department of Orthopedics & Microsurgery, Guangdong Second Provincial General Hospital, Guangzhou 510317, China
J Healthc Eng. 2017;2017. doi: 10.1155/2017/3571267.
Bone fracture is a global healthcare issue for high rates of delayed healing and nonunions. Although n-3 polyunsaturated fatty acid (PUFA) is considered as a beneficial factor for bone metabolism, only few studies till date focused on the effects of n-3 PUFAs on fracture healing. In this study, we investigated the effect of endogenous n-3 PUFAs on fracture healing by measuring femur fracture repair in both fat-1 transgenic mice and WT mice. Proximal femoral fracture model was established in fat-1 transgenic mice and WT mice, respectively, and then the fracture was analyzed by using X-ray, micro-computed tomography (micro-CT), and histological assessment at 7, 14, 21, 28, and 35 days after fixation. The results showed that compared with WT mice, fat-1 mice exhibited acceleration in fracture healing through radiographic and histological analysis (18–21 days versus 21–28 days postfracture). Meanwhile, X-ray and micro-CT analysis that showed better remodeling callus formation were in the fat-1 group compared to WT group. Furthermore, histological analysis revealed that endogenous n-3 PUFAs promoted local endochondral ossification and accelerated the remodeling of calcified calluses after fracture. In conclusion, the present study indicated that endogenously produced n-3 PUFAs promote fracture healing process and accelerate bone remodeling in mice, and supplementation of n-3 PUFAs was positively associated with fracture healing.
骨折是一个全球性的医疗保健问题，因为其延迟愈合和不愈合的发生率很高。尽管n-3多不饱和脂肪酸（PUFA）被认为是骨代谢的有益因素，但迄今为止只有少数研究关注n-3多不饱和脂肪酸对骨折愈合的影响。在本研究中，我们通过测量fat-1转基因小鼠和野生型（WT）小鼠的股骨骨折修复情况，研究内源性n-3多不饱和脂肪酸对骨折愈合的影响。分别在fat-1转基因小鼠和WT小鼠中建立股骨近端骨折模型，然后在固定后第7、14、21、28和35天通过X射线、显微计算机断层扫描（micro-CT）和组织学评估对骨折进行分析。结果显示，通过影像学和组织学分析，与WT小鼠相比，fat-1小鼠的骨折愈合加速（骨折后18 - 21天对21 - 28天）。同时，与WT组相比，fat-1组的X射线和micro-CT分析显示出更好的骨痂重塑形成。此外，组织学分析表明，内源性n-3多不饱和脂肪酸促进局部软骨内成骨，并加速骨折后钙化骨痂的重塑。总之，本研究表明，内源性产生的n-3多不饱和脂肪酸促进小鼠骨折愈合过程并加速骨重塑，补充n-3多不饱和脂肪酸与骨折愈合呈正相关。
