Hartel S, Hanski C, Neumeier R, Gossrau R, Reutter W
Institut für Molekularbiologie und Biochemie, Freien Universität, Berlin.
Adv Exp Med Biol. 1988;240:207-14. doi: 10.1007/978-1-4613-1057-0_25.
Nine monoclonal antibodies directed against DPP IV from rat liver plasma membranes were obtained. They recognized four different epitopes (A, B, C and D) of the enzyme. The epitopes A, B and C were located on the outside of the hepatocyte plasma membrane and were shared by DPP IV from hepatoma plasma membrane and the soluble form. Epitope D appeared to be partly inserted in the membrane and was found exclusively in the liver. Epitopes A, B and C and DPP IV revealed by histochemical means showed similar distribution patterns on frozen sections of various rat tissues, while epitope D did not show such a correlation. DPP IV is inserted in liver plasma membrane by two different mechanisms, one being phospholipase C-sensitive, while in hepatoma the enzyme is anchored in this membrane by a phospholipase C-resistant mechanism only.
获得了九种针对大鼠肝细胞膜二肽基肽酶IV（DPP IV）的单克隆抗体。它们识别该酶的四个不同表位（A、B、C和D）。表位A、B和C位于肝细胞质膜外侧，肝癌细胞质膜的DPP IV和可溶性形式也共有这些表位。表位D似乎部分插入膜中，且仅在肝脏中发现。通过组织化学方法显示的表位A、B、C和DPP IV在各种大鼠组织的冰冻切片上呈现相似的分布模式，而表位D则未显示出这种相关性。DPP IV通过两种不同机制插入肝细胞膜，一种对磷脂酶C敏感，而在肝癌中该酶仅通过对磷脂酶C有抗性的机制锚定在该膜上。
