心包和心外膜脂肪组织中米色脂肪细胞的分化可诱导心房颤动的发生。
The differentiation of beige adipocyte in pericardial and epicardial adipose tissues induces atrial fibrillation development.
机构信息
Department of Cardiothoracic Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
出版信息
Eur Rev Med Pharmacol Sci. 2017 Oct;21(19):4398-4405.
OBJECTIVE
Growing evidence has identified that excessive accumulation of pericardial adipose tissues (PAT) and epicardial adipose tissues (EAT) is associated with atrial fibrillation (AF) development. Moreover, beige adipocytes, present in PAT and EAT, have been proved beneficial in consumption of fatty acid and promotion of weight lose by nonshivering thermogenesis. The objective of this prospective, observational study was to reveal the potential association between beige adipocytes and AF development.
PATIENTS AND METHODS
Fat tissues from subcutaneous adipose tissue (SAT), PAT and EAT were obtained from 70 AF and 30 sinus rhythm patients. Hematoxylin and eosin (H&E) staining were performed to analyze morphological changes in fat tissues. Real-time PCR was performed to identify mRNA expression of unique uncoupling protein-1 (UCP-1). Western blotting and immunohistochemistry (IHC) were performed to determine protein expression of UCP-1.
RESULTS
Our results indicated that pericardial and epicardial adipocytes in AF patients demonstrated white-like change tendency and had lower expression of UCP-1 when compared to sinus rhythm patients. Additionally, the decrease of UCP-1 mRNA expression in PAT and EAT, together with LA enlargement, were independent risk factors of AF. Further, UCP-1 mRNA expression in EAT, but not in PAT, have a significant correlation with LA diameter. The function of nonshivering thermogenesis in PAT and EAT was impaired in AF patients, and this dysfunction in EAT had a great correlation with LA dilation.
CONCLUSIONS
Our data provide a new therapeutic target for LA remodeling and AF treatment.
目的
越来越多的证据表明，心包脂肪组织（PAT）和心外膜脂肪组织（EAT）的过度积累与心房颤动（AF）的发展有关。此外，存在于 PAT 和 EAT 中的米色脂肪细胞已被证明有利于脂肪酸的消耗和非颤抖性产热促进体重减轻。本前瞻性观察研究的目的是揭示米色脂肪细胞与 AF 发展之间的潜在关联。
患者和方法
从 70 例 AF 患者和 30 例窦性节律患者的皮下脂肪组织（SAT）、PAT 和 EAT 中获取脂肪组织。进行苏木精和伊红（H&E）染色以分析脂肪组织的形态变化。进行实时 PCR 以鉴定独特解偶联蛋白 1（UCP-1）的 mRNA 表达。进行 Western blot 和免疫组织化学（IHC）以确定 UCP-1 的蛋白表达。
结果
我们的结果表明，与窦性节律患者相比，AF 患者的心包和心外膜脂肪细胞表现出白色样变化趋势，并且 UCP-1 的表达较低。此外，PAT 和 EAT 中 UCP-1mRNA 表达的降低，以及左心房增大，是 AF 的独立危险因素。此外，EAT 中的 UCP-1mRNA 表达，而不是 PAT 中的表达，与 LA 直径有显著相关性。PAT 和 EAT 中的非颤抖性产热功能在 AF 患者中受损，而 EAT 中的这种功能障碍与 LA 扩张密切相关。
结论
我们的数据为左心房重构和 AF 治疗提供了新的治疗靶点。
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