通过RNA:RNA原位杂交检测感染蚊子体内的黄热病毒核酸。

Detection of yellow fever virus nucleic acid in infected mosquitoes by RNA:RNA in situ hybridization.

作者信息

Ballinger M E, Rice C M, Miller B R

机构信息

Division of Vector-Borne Viral Diseases, Centers for Disease Control, Fort Collins, Colorado 80522.

出版信息

Mol Cell Probes. 1988 Dec;2(4):331-8. doi: 10.1016/0890-8508(88)90016-3.

DOI:10.1016/0890-8508(88)90016-3

PMID:2907776

Abstract

An in situ hybridization technique was developed for the strand-specific detection of yellow fever virus (YFV) RNA. An 35S-labeled, transcribed RNA probe was used to detect positive-sense polarity YFV genomic RNA in infected C6/36 (Aedes albopictus) cells, dissected mosquito tissues, and sections of plastic-embedded, YFV-infected Aedes aegypti mosquitoes. Mosquito tissues fixed in buffered Formalin retained morphological integrity. The low concentrations of probe used yielded high specific signal on infected specimens and low background signal on uninfected specimens.

摘要

已开发出一种原位杂交技术，用于黄热病毒（YFV）RNA的链特异性检测。使用35S标记的转录RNA探针，检测受感染的C6/36（白纹伊蚊）细胞、解剖的蚊虫组织以及塑料包埋的感染YFV的埃及伊蚊蚊虫切片中的正义极性YFV基因组RNA。用缓冲福尔马林固定的蚊虫组织保持了形态完整性。所使用的低浓度探针在感染标本上产生高特异性信号，在未感染标本上产生低背景信号。