Azqueta Amaya, Rundén-Pran Elisa, Elje Elisabeth, Nicolaissen Bjørn, Berg Kristiane Haug, Smeringaiova Ingrida, Jirsova Katerina, Collins Andrew R
Department of Nutrition, Food Science and Toxicology, Schools of Pharmacy and Sciences, University of Navarra, Irunlarrea, Pamplona, Spain.
IdiSNA, Navarra Institute for Health Research.
Mutagenesis. 2018 Feb 24;33(1):21-24. doi: 10.1093/mutage/gex025.
The human eye is relatively unexplored as a source of cells for investigating DNA damage. There have been some clinical studies, using cells from surgically removed tissues, and altered DNA bases as well as strand breaks have been measured using the comet assay. Tissues examined include corneal epithelium and endothelium, lens capsule, iris and retinal pigment epithelium. For the purpose of biomonitoring for exposure to potential mutagens in the environment, the eye-relatively unprotected as it is compared with the skin-would be a valuable object for study; non-invasive techniques exist to collect lachrymal duct cells from tears, or cells from the ocular surface by impression cytology, and these methods should be further developed and validated.
作为研究DNA损伤的细胞来源，人眼相对未被充分探索。已经有一些临床研究，使用手术切除组织中的细胞，并通过彗星试验测量了改变的DNA碱基以及链断裂。所检查的组织包括角膜上皮和内皮、晶状体囊、虹膜和视网膜色素上皮。出于对环境中潜在诱变剂暴露进行生物监测的目的，与皮肤相比相对未受保护的眼睛将是一个有价值的研究对象；存在从泪液中收集泪道细胞或通过印迹细胞学从眼表收集细胞的非侵入性技术，这些方法应进一步开发和验证。
