Bankvall M, Jontell M, Wold A, Östman S
Department of Oral Medicine & Pathology, Institute of Odontology, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Department of Infectious Diseases, Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Scand J Immunol. 2018 Jan;87(1):15-27. doi: 10.1111/sji.12625. Epub 2017 Dec 5.
Defining the immune cells within the naso-oropharyngeal-associated lymphoid tissues would promote the development of efficient orally and nasally delivered immunotherapies. The aim was to compare murine antigen-presenting cells (APCs) and T cell subsets in the nose-associated lymphoid tissues (NALT), cervical lymph nodes (CLN), mesenteric lymph nodes (MLN) and peripheral lymph nodes (PLN) using flow cytometry and in vitro proliferation assays. Overall, the NALT contained a higher proportion of APCs and a lower proportion of T cells compared to the CLN, MLN and PLN. The APCs of the NALT more often belonged to the CD11c CD11b and the CD11c CD11b subsets as compared to the other sites. Both of these APC populations showed little sign of activation, that is low expression of the markers CD40, CD86 and IAd. Instead, the APCs of the NALT more often co-expressed CX3CR1 and CD206, markers associated with a tolerogenic function. No increase in the proportion of regulatory T cells was observed in the NALT. Instead, the T cells frequently exhibited a memory/effector phenotype, expressing the homing markers α4β7, CCR4 and CCR9, but rarely the naïve phenotype cell surface marker CD45RB. In contrast, the T cells at the other sites were mostly of the naïve phenotype. In addition, cells from the NALT did not proliferate upon in vitro stimulation with Con A, whereas the cells from the other sites did. Taken together, these results suggest that the NALT is primarily an effector site rather than one for activation and differentiation, despite it being regarded as a site of induction.
明确鼻咽相关淋巴组织中的免疫细胞，将推动高效的口服和鼻用免疫疗法的发展。目的是使用流式细胞术和体外增殖试验，比较鼻相关淋巴组织（NALT）、颈淋巴结（CLN）、肠系膜淋巴结（MLN）和外周淋巴结（PLN）中的小鼠抗原呈递细胞（APC）和T细胞亚群。总体而言，与CLN、MLN和PLN相比，NALT中APC的比例更高，T细胞的比例更低。与其他部位相比，NALT中的APC更常属于CD11c⁺CD11b⁻和CD11c⁺CD11b⁺亚群。这两种APC群体几乎没有激活迹象，即CD40、CD86和IAd标志物的低表达。相反，NALT中的APC更常共表达CX3CR1和CD206，这两种标志物与致耐受性功能相关。在NALT中未观察到调节性T细胞比例增加。相反，T细胞经常表现出记忆/效应表型，表达归巢标志物α4β7、CCR4和CCR9，但很少表达幼稚表型细胞表面标志物CD45RB。相比之下，其他部位的T细胞大多为幼稚表型。此外，NALT中的细胞在体外受到伴刀豆球蛋白A刺激时不增殖，而其他部位的细胞则增殖。综上所述，这些结果表明，尽管NALT被视为诱导部位，但其主要是效应部位而非激活和分化部位。
