Nanoscience Research Laboratory, School of Nano Science and Technology, National Institute of Technology, Calicut 673601, India.
Nanoscience Research Laboratory, School of Nano Science and Technology, National Institute of Technology, Calicut 673601, India; CSIR-Central Electrochemical Research Institute, Karaikudi 630006, India.
Biosens Bioelectron. 2018 Mar 15;101:199-205. doi: 10.1016/j.bios.2017.10.030. Epub 2017 Oct 16.
We have constructed an aptamer immobilized gold atomic cluster mediated, ultrasensitive electrochemical biosensor (Apt/AuAC/Au) for LPS detection without any additional signal amplification strategy. The aptamer self-assemble onto the gold atomic clusters makes Apt/AuAC/Au an excellent platform for the LPS detection. Differential pulse voltammetry and EIS were used for the quantitative LPS detection. The Apt/AuAC/Au sensor offers an ultrasensitive and selective detection of LPS down to 7.94 × 10M level with a wide dynamic range from 0.01 attomolar to 1pM. The sensor exhibited excellent selectivity and stability. The real sample analysis was performed by spiking the diluted insulin sample with various concentration of LPS and obtained recovery within 2% error value. The sensor is found to be more sensitive than most of the literature reports. The simple and easy way of construction of this sensor provides an efficient and promising detection of an even trace amount of LPS.
我们构建了一种适体固定金原子簇介导的超灵敏电化学生物传感器（Apt/AuAC/Au），用于 LPS 的检测，而无需任何额外的信号放大策略。适体自组装到金原子簇上，使 Apt/AuAC/Au 成为 LPS 检测的绝佳平台。差分脉冲伏安法和 EIS 用于定量 LPS 检测。Apt/AuAC/Au 传感器对 LPS 进行超灵敏和选择性检测，检测下限低至 7.94×10^-10 M 级，动态范围从 0.01 飞摩尔到 1 pM。传感器表现出优异的选择性和稳定性。通过将稀释的胰岛素样品中混入不同浓度的 LPS 进行实际样品分析，得到的回收率在 2%误差值以内。该传感器的灵敏度比大多数文献报道的都要高。这种传感器的构建方法简单易行，为检测痕量 LPS 提供了一种高效、有前景的方法。
