Menéndez Fernández-Miranda Candela, Fernández-Suarez Jonathan, Rodríguez-Pérez Mercedes, Menéndez Fernández-Miranda Pablo, Vázquez Fernando, Boga Ribeiro Jose A, Rodríguez-Guardado Azucena
Tropical Medicine Unit, Hospital Universitario Central de Asturias, Oviedo, Spain.
Microbiology Unit, Hospital Universitario Central de Asturias, Oviedo, Spain.
Enferm Infecc Microbiol Clin (Engl Ed). 2018 Aug-Sep;36(7):423-427. doi: 10.1016/j.eimc.2017.08.004. Epub 2017 Oct 25.
The results of a study on the household contacts of patients with D. fragilis infection are presented.
A prospective, descriptive study was carried out on all Dientamoeba fragilis-infected patients treated at the Tropical Medicine Unit of Hospital Universitario Central de Asturias between 2012- 2017 and their household contacts. Three stool samples per patient and three stool samples from each of their household contacts were concentrated and analysed. Polymerase chain reaction (PCR) was used to detect the presence of D. fragilis in all stool samples. Co-infection with E. vermicularis was studied in both groups. Patients and contacts who failed to deliver one or more samples for diagnosis and patients without household contacts were excluded.
44 Patients infected with D. fragilis, as well as their 97 household contacts were enrolled. 50.5% of household contacts had a positive PCR for D. fragilis. 20 were also coinfected with E. vermicularis. The presence of infection was significantly more frequent in patients with children (34/15 versus 24/24; p= 0.064; OR: 2.267 [0.988-5.199]), E. vermicularis infection in the children being 20/29 versus 0/48 (p=0.0001), and in another family member being 29/20 versus 15/33 (p=0.008; OR: 3.190 [1.384-7.352]).
The prevalence of D. fragilis infection in household contacts was high. It was associated with the presence of children in the family nucleus and coinfection with E. vermicularis irrespective of gender, age, rural area or contact with animals.
本文展示了一项关于脆弱双核阿米巴感染患者家庭接触者的研究结果。
对2012年至2017年间在阿斯图里亚斯中央大学医院热带医学科接受治疗的所有脆弱双核阿米巴感染患者及其家庭接触者进行了一项前瞻性描述性研究。每位患者采集三份粪便样本，其每位家庭接触者也采集三份粪便样本，进行浓缩和分析。采用聚合酶链反应（PCR）检测所有粪便样本中脆弱双核阿米巴的存在情况。对两组患者同时研究了与蠕形住肠线虫的合并感染情况。未提供一份或多份诊断样本的患者和接触者以及没有家庭接触者的患者被排除在外。
纳入了44例脆弱双核阿米巴感染患者及其97名家庭接触者。50.5%的家庭接触者PCR检测脆弱双核阿米巴呈阳性。其中20人还合并感染了蠕形住肠线虫。有儿童的患者中感染的发生率明显更高（34/15对比24/24；p = 0.064；OR：2.267 [0.988 - 5.199]），儿童中蠕形住肠线虫感染率为20/29对比0/48（p = 0.0001），另一名家庭成员中为29/20对比15/33（p = 0.008；OR：3.190 [1.384 - 7.352]）。
家庭接触者中脆弱双核阿米巴感染的患病率较高。它与家庭核心成员中有儿童以及与蠕形住肠线虫合并感染有关，与性别、年龄、农村地区或与动物接触无关。
