乏氧条件下 ALA 光动力疗法诱导结肠癌血管内皮生长因子分泌。
Secretion of the angiogenic factor VEGF after photodynamic therapy with ALA under hypoxia-like conditions in colon cancer cells.
机构信息
School of Medicine with the Division of Dentistry in Zabrze, Department of Internal Diseases, Angiology and Physical Medicine, Center for Laser Diagnostics and Therapy, Medical University of Silesia, Batorego Street 15, 41-902 Bytom, Poland.
Specialist Hospital No2, Department of Internal Diseases, Angiology and Physical Medicine, Center for Laser Diagnostics and Therapy, Batorego Street 15, 41-902 Bytom, Poland.
出版信息
Photodiagnosis Photodyn Ther. 2018 Mar;21:16-18. doi: 10.1016/j.pdpdt.2017.10.020. Epub 2017 Oct 24.
BACKGROUND
Photodynamic therapy (PDT), eliminates not only the tumor, but also modulates signaling factors release, e.g. vascular endothelial growth factor (VEGF), which plays a crucial role in cancer progression. Assessment of the VEGF-secreting activity of resistant colon cancer cells in different degree of malignancy: SW480 and SW620 under hypoxia-like conditions during δ- aminolevulinic acid (ALA) PDT was the objective of our study.
METHODS
The colon cancer cell lines SW480 and SW620 were treated in sublethal doses with ALA PDT in hypoxia- like conditions with cobalt chloride (CoCl). To assess cell viability, MTT assays were performed and the discrimination of the cell death mode was monitored via fluorescence microscopy. The cells cytotoxicity using LDH test was assessed. Determination of VEGF was carried out using the Bio- Plex Assay Pro™ kit on the Bio- Plex Suspension Array System.
RESULTS
ALA PDT used in sublethal doses decreases release of VEGF in more aggressively growing SW620 colon cancer cell line in hypoxia-like conditions. In addition the level of secretion of VEGF in SW620 was much higher than in SW480 cells, which correlates with the grade of aggressive growth of colon cancer cells.
CONCLUSION
Our outcomes offer evidence, that in hypoxia mimic condition sublethal ALA-PDT- mediated VEGF inhibition could be clinically relevant.
背景
光动力疗法（PDT）不仅可以消除肿瘤，还可以调节信号因子的释放，例如血管内皮生长因子（VEGF），它在癌症进展中起着至关重要的作用。本研究旨在评估不同恶性程度的耐结肠癌细胞在δ-氨基酮戊酸（ALA）PDT 缺氧样条件下 VEGF 分泌活性：SW480 和 SW620。
方法
用钴氯化物（CoCl）在缺氧样条件下用亚致死剂量的 ALA PDT 处理结肠癌细胞系 SW480 和 SW620。为了评估细胞活力，进行了 MTT 测定，并通过荧光显微镜监测细胞死亡模式的区分。使用 LDH 试验评估细胞毒性。使用 Bio- Plex Assay Pro™试剂盒在 Bio- Plex 悬浮阵列系统上测定 VEGF 的含量。
结果
亚致死剂量的 ALA PDT 在缺氧样条件下降低了更具侵袭性生长的 SW620 结肠癌细胞系中 VEGF 的释放。此外，SW620 中 VEGF 的分泌水平明显高于 SW480 细胞，这与结肠癌细胞侵袭性生长的程度相关。
结论
我们的研究结果表明，在缺氧模拟条件下，亚致死剂量的 ALA-PDT 介导的 VEGF 抑制可能具有临床意义。
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