猪圆环病毒3型间接ELISA方法的建立与应用
Establishment and application of an indirect ELISA for porcine circovirus 3.
作者信息
Deng Junhua, Li Xiangdong, Zheng DingDing, Wang Yanwei, Chen Lingyan, Song Huanhuan, Wang Tongyan, Huang Yuxin, Pang Wenqiang, Tian Kegong
机构信息
National Research Center for Veterinary Medicine, High-Tech District, Luoyang, China.
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.
出版信息
Arch Virol. 2018 Feb;163(2):479-482. doi: 10.1007/s00705-017-3607-7. Epub 2017 Oct 27.
Porcine circovirus type 3 (PCV3) was initially reported in 2016 in the United States of America. Since then, the virus has been detected on swine farms in Poland, South Korea, and China using PCR. However, a serological survey of PCV3 in pig populations has never been conducted. In this study, for the first time, we established an indirect enzyme-linked immunosorbent (ELISA) assay and performed a national retrospective serological survey for PCV3. Our results showed that the PCV3-postive rate increased from 22.35% to 51.88% between 2015 and 2017. The above results suggest PCV3 has spread widely in China with increased positive rates since 2015.
猪圆环病毒3型（PCV3）于2016年首次在美国被报道。从那时起，通过聚合酶链反应（PCR）在波兰、韩国和中国的养猪场中检测到了该病毒。然而，从未对猪群进行过PCV3的血清学调查。在本研究中，我们首次建立了间接酶联免疫吸附测定（ELISA）法，并对PCV3进行了全国性回顾性血清学调查。我们的结果表明，2015年至2017年间，PCV3阳性率从22.35%上升至51.88%。上述结果表明，自2015年以来，PCV3在中国广泛传播，阳性率不断上升。
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