Krishnavajhala Haritha Ramya, Williams Jacqueline, Heidner Hans
Department of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX, 78006, USA.
Arch Virol. 2018 Feb;163(2):483-488. doi: 10.1007/s00705-017-3578-8. Epub 2017 Oct 27.
The 23-residue external domain of the influenza A virus M2 protein (M2e) has significant potential as a vaccine antigen. Here, we describe the construction and characterization of an M2e-modified Sindbis virus designated E2S1-M2e. E2S1-M2e virions contain M2e as an N-terminal extension of the E2 glycoprotein and therefore express 240 copies of the M2e peptide on their surface. The E2S1-M2e virus expressed M2e in an accessible and immunogenic form and induced M2e-specific antibodies when administered to mice. Mice that received an intranasal vaccination with E2S1-M2e were protected against a lethal challenge with a virulent, mouse-adapted strain of influenza A virus.
甲型流感病毒M2蛋白（M2e）的23个氨基酸的外部结构域作为疫苗抗原有很大潜力。在此，我们描述了一种名为E2S1-M2e的M2e修饰的辛德毕斯病毒的构建和特性。E2S1-M2e病毒粒子含有M2e作为E2糖蛋白的N端延伸，因此在其表面表达240个拷贝的M2e肽。E2S1-M2e病毒以可及且具有免疫原性的形式表达M2e，并在给小鼠接种时诱导产生M2e特异性抗体。接受E2S1-M2e鼻内接种的小鼠在受到甲型流感病毒强毒株、小鼠适应株的致死性攻击时受到保护。
