Angelini Riccardo, Cona Alessandra, Tavladoraki Paraskevi
Department of Science, University "Roma Tre", Viale Guglielmo Marconi 446, 00146, Rome, Italy.
Methods Mol Biol. 2018;1694:129-139. doi: 10.1007/978-1-4939-7398-9_13.
Copper amine oxidases (CuAOs) involved in polyamine catabolism are emerging as physiologically relevant enzymes for their involvement in plant growth, differentiation and defence responses to biotic and abiotic stress. In this chapter, we describe two spectrophotometric and one polarographic method for determining CuAO activity in plant tissues. Some aspects related to cell wall association of apoplastic CuAOs and possible interference of plant metabolites with the enzymatic activity assays are also considered.
参与多胺分解代谢的铜胺氧化酶（CuAOs）正逐渐成为具有生理相关性的酶，因为它们参与植物生长、分化以及对生物和非生物胁迫的防御反应。在本章中，我们描述了两种分光光度法和一种极谱法来测定植物组织中的CuAO活性。还考虑了与质外体CuAOs细胞壁结合相关的一些方面以及植物代谢物对酶活性测定可能产生的干扰。
