Pereira Claudio A, Sayé Melisa, Reigada Chantal, Miranda Mariana R
Laboratorio de Parasitología Molecular, Instituto de Investigaciones Médicas "A. Lanari", IDIM-CONICET, Universidad de Buenos Aires, Combatientes de Malvinas, 3150, Buenos Aires, Argentina.
Methods Mol Biol. 2018;1694:225-232. doi: 10.1007/978-1-4939-7398-9_21.
Transport systems are key processes in every living organism: they allow the entry of all essential nutrients into the cell and its compartments and regulate the intracellular concentrations of metabolites. The transport of cell nutrients represents the first step of many metabolic routes and may also regulate such processes. They are also responsible for reaching the effective intracellular concentration of therapeutic drugs and some mechanisms of resistance and tolerance also depend on them. However, the common techniques used to evaluate the metabolites transport in different cells types are not easy to carry out and require extensive training. In this chapter, we report detailed protocols and tips about the expression of transporters, different activity assays and transporter kinetics determination.
