Analysis of DNA Methylation Content and Patterns in Plants.

DNA methylation is an epigenetic modification, which contributes to the regulation of gene expression and chromatin organization, and thus plays a role in many aspects of plant life. Here we present three methods for the detection of DNA methylation in plant tissues: high performance liquid chromatography, methylation-sensitive restriction digest followed by quantitative PCR and bisulfite conversion followed by single read sequencing. These methods are complementary and allow analysis of DNA methylation in samples from both model and non-model plant species.