Atanasov Kostadin E, Liu Changxin, Tiburcio Antonio F, Alcázar Rubén
Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Barcelona, Spain.
Methods Mol Biol. 2018;1694:343-346. doi: 10.1007/978-1-4939-7398-9_29.
In the recent years, genetic engineering of polyamine biosynthetic genes has provided evidence for their involvement in plant stress responses and different aspects of plant development. Such approaches are being complemented with the use of reverse genetics, in which mutants affected on a particular trait, tightly associated with polyamines, are isolated and the causal genes mapped. Reverse genetics enables the identification of novel genes in the polyamine pathway, which may be involved in downstream signaling, transport, homeostasis, or perception. Here, we describe a basic protocol for the generation of ethyl methanesulfonate (EMS) mutagenized populations of Arabidopsis thaliana for its use in reverse genetics applied to polyamines.
近年来，多胺生物合成基因的基因工程为其参与植物应激反应和植物发育的不同方面提供了证据。此类方法正通过反向遗传学的应用得到补充，在反向遗传学中，分离出在与多胺紧密相关的特定性状上受到影响的突变体，并对因果基因进行定位。反向遗传学能够鉴定多胺途径中的新基因，这些基因可能参与下游信号传导、运输、稳态或感知过程。在此，我们描述了一种用于生成拟南芥甲基磺酸乙酯（EMS）诱变群体的基本方案，该方案用于应用于多胺的反向遗传学研究。
