多胺突变体的非生物胁迫表型分析

Abiotic Stress Phenotyping of Polyamine Mutants.

作者信息

Berberich Thomas, Sagor G H M, Kusano Tomonobu

机构信息

Laboratory Center, Senckenberg Biodiversity and Climate Research Center (SBiK-F), Georg-Voigt-Str. 14-16, 60325, Frankfurt am Main, Germany.

Department of Genetics & Plant Breeding, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

出版信息

Methods Mol Biol. 2018;1694:389-403. doi: 10.1007/978-1-4939-7398-9_32.

DOI:10.1007/978-1-4939-7398-9_32

PMID:29080182

Abstract

Plant mutants in polyamine pathway genes are ideal for investigating their roles in stress responses. Here we describe easy-to-perform methods for phenotyping Arabidopsis mutants under abiotic stress. These include measurements of root growth, chlorophyll content, water loss, electrolyte leakage, and content of the reactive oxygen species hydrogen peroxide (HO) and superoxide anion (O-). Growth of Arabidopsis seedlings is described that enables transfer to different media for stress treatment without damaging roots.

摘要

多胺途径基因的植物突变体是研究它们在应激反应中作用的理想材料。在此，我们描述了在非生物胁迫下对拟南芥突变体进行表型分析的简便方法。这些方法包括测量根生长、叶绿素含量、水分流失、电解质渗漏以及活性氧过氧化氢（HO）和超氧阴离子（O-）的含量。还描述了拟南芥幼苗的生长情况，这种生长方式能够在不损伤根系的情况下将其转移到不同培养基上进行胁迫处理。