葎草素介导的 GLUT1 抑制人舌鳞状细胞癌的生长。
Plumbagin-mediating GLUT1 suppresses the growth of human tongue squamous cell carcinoma.
机构信息
Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, China.
Research Institute of Digestive Diseases and Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, China.
出版信息
Oral Dis. 2018 Sep;24(6):920-929. doi: 10.1111/odi.12799. Epub 2018 Jul 11.
OBJECTIVE
The purpose of this study was to investigate the clinical and histopathological characteristics of GLUT1 in human tongue squamous cell carcinoma (TSCC) and the role of plumbagin (PLB)-mediating GLUT1 in the growth of TSCC.
SUBJECTS AND METHODS
Forty-five cases of TSCC samples were collected and the expression and location of GLUT1 was analyzed. The role and mechanism of PLB meditating GLUT1 in the inhibitory growth of human TSCC cell line CAL27 were investigated in vitro and vivo.
RESULTS
The expression of GLUT1 was observed in all samples of human TSCC by immunohistochemical staining. GLUT1 expression was significantly correlated with lymph node metastasis and clinical stage in TSCC. PLB treatment decreased cell viability and colony formation, and increased cell apoptosis in association with the downregulation of GLUT1 via inhibiting PI3K/Akt pathway in vitro and PLB suppressed tumor growth in correlation with downregulation of GLUT1, compared with control group in vivo.
CONCLUSIONS
The findings demonstrated a novel anti-cancer mechanism of PLB, inhibitory TSCC growth via suppressing PI3K/Akt/GLUT1 pathway, which will supply a theoretical basis for PLB to treat TSCC.
目的
本研究旨在探讨葡萄糖转运蛋白 1（GLUT1）在人舌鳞状细胞癌（TSCC）中的临床和组织病理学特征，以及白花丹醌（PLB）介导的 GLUT1 在 TSCC 生长中的作用。
材料和方法
收集 45 例 TSCC 样本，分析 GLUT1 的表达和定位。在体外和体内研究 PLB 介导 GLUT1 对人舌鳞癌细胞系 CAL27 生长抑制的作用和机制。
结果
免疫组织化学染色显示，所有 TSCC 样本中均观察到 GLUT1 的表达。GLUT1 的表达与 TSCC 的淋巴结转移和临床分期显著相关。PLB 处理通过抑制 PI3K/Akt 通路，降低 GLUT1 的表达，降低细胞活力和集落形成，增加细胞凋亡，与对照组相比，体内 PLB 抑制肿瘤生长与下调 GLUT1 相关。
结论
这些发现表明了 PLB 的一种新的抗癌机制，通过抑制 PI3K/Akt/GLUT1 通路抑制 TSCC 生长，为 PLB 治疗 TSCC 提供了理论基础。
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