刻纹花蜱线粒体基因组的测序与比较分析
Sequencing and comparative analysis of the Amblyomma sculptum mitogenome.
作者信息
de Lima Paulo Henrique Costa, Barcelos Rafael Mazioli, Klein Raphael Contelli, Vidigal Pedro Marcus Pereira, Montandon Carlos Emmanuel, Fabres-Klein Mary Hellen, Dergam Jorge Abdala, Mafra Cláudio
机构信息
Federal University of Viçosa, Biochemistry and Molecular Biology Department, Minas Gerais, Brazil; Federal Institute of Education, Science and Technology of Amazonas, Amazonas, Brazil.
Federal University of Viçosa, Biochemistry and Molecular Biology Department, Minas Gerais, Brazil.
出版信息
Vet Parasitol. 2017 Nov 30;247:121-128. doi: 10.1016/j.vetpar.2017.10.007. Epub 2017 Oct 16.
The mitogenome of Amblyomma sculptum was sequenced, providing important information for understanding the evolutionary relationships among species of the A. cajennense complex. The mitochondrial genome has a circular structure with 37 genes, including 13 coding DNA sequences, two ribosomal RNA genes (12S rRNA and 16S rRNA) and 22 tRNA genes. Comparative analysis with the mitogenomes of six reference species of the genus Amblyomma revealed that the ND5 gene, which is related to energy metabolism, and control regions 1 and 2 of the mitogenomes have polymorphisms that can be exploited as molecular markers to differentiate A. sculptum from other tick species in the Amblyomma cajennense complex as well as other Amblyomma species.
对刻纹花蜱的线粒体基因组进行了测序，为理解卡延花蜱复合体物种间的进化关系提供了重要信息。线粒体基因组具有环状结构，包含37个基因，其中包括13个编码DNA序列、两个核糖体RNA基因（12S rRNA和16S rRNA）以及22个tRNA基因。与花蜱属六个参考物种的线粒体基因组进行比较分析发现，与能量代谢相关的ND5基因以及线粒体基因组的控制区1和控制区2具有多态性，这些多态性可作为分子标记，用于区分刻纹花蜱与卡延花蜱复合体中的其他蜱种以及其他花蜱物种。
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