[Detection of gene by dual allele-specific real-time polymerase chain reaction in patients with trichlorethylene-induced dermatitis].

To investigate the detection of a human leukocyte antigen-B (HLA-B) allele by dual allele-specific real-time polymerase chain reaction (PCR) in patients with trichlorethylene-induced dermatitis. A total of 20 patients with trichlorethylene-induced dermatitis who were admitted and treated from January 2014 to October 2016 were enrolled as case group, and 20 persons who underwent physical examination from January to October, 2016 were enrolled as control group. Peripheral cubital venous blood samples were collected from all subjects, and dual allele-specific real-time PCR was used to detect the gene. The two groups were compared in terms of the proportion of subjects carrying gene. There were no significant differences between the case group and the control group in median age (25.0 years 27.0 years, =0.30, >0.05) and the proportion of male subjects (60.0% 70.0%, χ(2)=0.44, >0.05) . The mean time of exposure to trichloroethylene was 30.8 days in the case group, while the subjects in the control group were not exposed to trichloroethylene. The case group had a significantly higher frequency of gene than the control group (80.0% 20.0%, χ(2)=14.40, <0.01) with an odds ratio of 16.00. Dual allele-specific real-time PCR can be used for detection of the gene in patients with trichlorethylene-induced dermatitis.