三氯乙烯所致药疹样皮炎与HLA - DMA和HLA - DMB的关系

[Relationship of trichloroethylene-induced medicamentosa like dermatitis to HLA-DMA and HLA-DMB].

作者信息

Yue Fei, Huang Han-lin, Huang Zhen-lie, Li Lai-yu, Liang Li-yan, Chen Xue-min

机构信息

Department of Environmental Hygiene, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

出版信息

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2006 May;24(5):263-5.

PMID:16737583

Abstract

OBJECTIVE

To investigate the susceptibility of trichloroethylene-induced medicamentosa like dermatitis by comparing the frequency of HLA-DMA and HLA-DMB in patients with trichloroethylene-induced medicamentosa like dermatitis and in normal controls.

METHODS

The DNA of lymphocytes in 61 patients with trichloroethylene-induced medicamentosa like dermatitis and in 60 people as the normal control were abstracted by using touchdown PCR amplification of HLA-DMA and HLA-DMB. Then through restriction fragment length polymorphism (RFLP) and sequence base typing, the alleles and genotypes were confirmed. The frequency of HLA-DMA and HLA-DMB in the two groups was compared.

RESULTS

The HLA-DMA0101 allele frequency in patients with trichloroethylene-induced medicamentosa like dermatitis was significantly higher than in the control group (71.3% vs 55.0%, P < 0.05). The allele frequency of HLA-DMA0103 was significantly higher in the patient group than in the control group (11.5% vs 3.3%, P < 0.05). The ratio of 0102 homozygotes of HLA-DMA0102 in the patient group was significantly higher than in the control group (25.0% vs 8.2%, P < 0.05). The ratio of 0102 heterozygotes of HLA-DMB0101 in the patient group was lower than in the control group (P < 0.05).

CONCLUSION

The polymorphisms of DMA may be related to the susceptibility of the patients with trichloroethylene-induced medicamentosa like dermatitis.

摘要

目的

通过比较三氯乙烯所致药疹样皮炎患者与正常对照者中HLA - DMA和HLA - DMB的频率，探讨三氯乙烯所致药疹样皮炎的易感性。

方法

采用降落式PCR扩增HLA - DMA和HLA - DMB，提取61例三氯乙烯所致药疹样皮炎患者及60例正常对照者淋巴细胞的DNA。然后通过限制性片段长度多态性（RFLP）和序列碱基分型确定等位基因和基因型。比较两组中HLA - DMA和HLA - DMB的频率。

结果

三氯乙烯所致药疹样皮炎患者中HLA - DMA0101等位基因频率显著高于对照组（71.3%对55.0%，P < 0.05）。患者组中HLA - DMA0103等位基因频率显著高于对照组（11.5%对3.3%，P < 0.05）。患者组中HLA - DMA0102的0102纯合子比例显著高于对照组（25.0%对8.2%，P < 0.05）。患者组中HLA - DMB0101的0102杂合子比例低于对照组（P < 0.05）。