Craig F F, Lackie J M, Parton R, Freer J H
Department of Microbiology, University of Glasgow, UK.
J Gen Microbiol. 1988 Aug;134(8):2201-11. doi: 10.1099/00221287-134-8-2201.
The effect of secreted virulence components of Bordetella pertussis on chemiluminescence (CL) of rabbit peritoneal neutrophils was determined with the chemotactic peptide N'-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP) or intact B. pertussis as the stimulus. Pertussis toxin (PT) inhibited the response to fMLP in a dose-dependent manner, although only after the neutrophils had been exposed to the toxin for greater than 15 min. Both filamentous haemagglutinin (FHA) and lipopolysaccharide (LPS) markedly enhanced the CL response to fMLP after greater than or equal to 15 min incubation with the neutrophils. Similar effects to those of B. pertussis LPS were also seen with smooth and rough LPS from Salmonella minnesota. With the lowest dose of each component which elicited a maximal effect on CL, the inhibitory effect of PT overrode the enhancing effect of FHA and B. pertussis LPS. Pre-incubation of neutrophils with PT, FHA or B. pertussis LPS caused a slight reduction in the subsequent CL response to virulent B. pertussis Tohama. Virulent (phase I, or X-mode) organisms of B. pertussis 18334 and B. pertussis Tohama induced greater neutrophil CL than their avirulent (C-mode) derivatives. There appeared to be an inverse correlation between bacterial hydrophilicity and the ability to induce neutrophil CL: X-mode bacteria were significantly less hydrophilic than C-mode organisms. Three mutants, the adenylate cyclase (AC)- and haemolysin (HLY)-deficient B. pertussis BP348, the FHA-deficient B. pertussis BP353, and the PT-deficient B. pertussis BP357, generated similar levels of CL and had similar hydrophilicity values. The hydrophilicity value of the avirulent mutant B. pertussis BP347 (deficient in AC, HLY, FHA and PT) and the CL induced by this strain were similar to those of B. pertussis C-mode organisms. Thus, the interaction of B. pertussis with neutrophils appears to be complex, reflecting both the alteration of leucocyte function by secreted virulence components of the organism and, in the absence of opsonins, the surface properties of the bacterium.
以趋化肽N'-甲酰基-L-甲硫氨酰-L-亮氨酰-L-苯丙氨酸（fMLP）或完整的百日咳博德特氏菌作为刺激物，测定了百日咳博德特氏菌分泌的毒力成分对兔腹膜中性粒细胞化学发光（CL）的影响。百日咳毒素（PT）以剂量依赖的方式抑制对fMLP的反应，不过只有在中性粒细胞暴露于该毒素超过15分钟后才会出现这种情况。丝状血凝素（FHA）和脂多糖（LPS）在与中性粒细胞孵育大于或等于15分钟后，均显著增强了对fMLP的CL反应。来自明尼苏达沙门氏菌的光滑型和粗糙型LPS也表现出与百日咳博德特氏菌LPS类似的效果。在每种成分引起CL最大效应的最低剂量下，PT的抑制作用超过了FHA和百日咳博德特氏菌LPS的增强作用。中性粒细胞预先与PT、FHA或百日咳博德特氏菌LPS孵育，会使随后对强毒株百日咳博德特氏菌Tohama的CL反应略有降低。百日咳博德特氏菌18334和百日咳博德特氏菌Tohama的强毒株（I相或X模式）比其无毒株（C模式）衍生物诱导的中性粒细胞CL更强。细菌的亲水性与其诱导中性粒细胞CL的能力之间似乎呈负相关：X模式细菌的亲水性明显低于C模式细菌。三个突变株，即腺苷酸环化酶（AC）和溶血素（HLY）缺陷的百日咳博德特氏菌BP348、FHA缺陷的百日咳博德特氏菌BP353以及PT缺陷的百日咳博德特氏菌BP357，产生的CL水平相似且亲水性值相近。无毒突变株百日咳博德特氏菌BP347（AC、HLY、FHA和PT均缺陷）的亲水性值以及该菌株诱导的CL与百日咳博德特氏菌C模式细菌相似。因此，百日咳博德特氏菌与中性粒细胞的相互作用似乎很复杂，既反映了该菌分泌的毒力成分对白细胞功能的改变，也反映了在缺乏调理素的情况下细菌的表面特性。
