Suzuki Y, Yoshimura F, Takahashi K, Tani H, Suzuki T
Department of Preventive Dentistry, Hokkaido University, Sapporo, Japan.
J Gen Microbiol. 1988 Oct;134(10):2713-20. doi: 10.1099/00221287-134-10-2713.
We screened 63 clinical isolates of Bacteroides gingivalis from eight different laboratories for the presence of fimbriae by negative staining and by immunological methods. Techniques used were bacterial agglutination, Ouchterlony immunodiffusion and Western immunoblotting analysis using rabbit anti-fimbriae and anti-fimbrilin sera raised against fimbriae and fimbrilin (a constituent protein of B. gingivalis fimbriae) from B. gingivalis strain 381. In 49 of the 51 strains tested, fimbriae were clearly detected by negative staining, and 30 (60%) of the fimbriate strains were positive in all three of the immunological assays. A total of 37 strains (75%) were positive by immunoblotting analysis, which was the most reliable of the serological methods used in this study. The study shows that the majority of B. gingivalis strains are fimbriate, and that these fimbriae are immunologically related to the fimbriae of B. gingivalis strain 381. Molecular heterogeneity of fimbrilin was discovered by the immunoblotting analysis, when different strains were compared. With most of the strains, including strain 381, the antifimbrilin serum reacted with a protein of apparent molecular mass 43 kDa, but with 15 strains the immuno-reactive protein had an apparent molecular mass of 46 kDa.
我们通过负染色和免疫方法，对来自八个不同实验室的63株牙龈拟杆菌临床分离株进行菌毛检测。所采用的技术包括细菌凝集试验、双向免疫扩散试验以及使用兔抗菌毛血清和抗菌毛蛋白血清的Western免疫印迹分析，这些血清是针对牙龈拟杆菌381株的菌毛和菌毛蛋白（牙龈拟杆菌菌毛的一种组成蛋白）制备的。在51株受试菌株中，49株通过负染色清晰检测到菌毛，其中30株（60%）菌毛阳性菌株在所有三种免疫检测中均呈阳性。共有37株（75%）通过免疫印迹分析呈阳性，免疫印迹分析是本研究中使用的血清学方法中最可靠的。该研究表明，大多数牙龈拟杆菌菌株有菌毛，且这些菌毛与牙龈拟杆菌381株的菌毛在免疫学上相关。通过免疫印迹分析比较不同菌株时，发现了菌毛蛋白的分子异质性。对于大多数菌株，包括381株，抗菌毛蛋白血清与表观分子量为43 kDa的一种蛋白发生反应，但有15株菌株的免疫反应性蛋白表观分子量为46 kDa。
