Bradford Samantha M, Mikula Eric R, Chai Dongyul, Brown Donald J, Juhasz Tibor, Jester James V
Department of Biomedical Engineering, University of California, Irvine, Irvine, CA, USA.
Department of Ophthalmology, 843 Health Sciences Road, University of California, Irvine, CA, 92697-4390, USA.
Biomed Opt Express. 2017 Sep 29;8(10):4788-4797. doi: 10.1364/BOE.8.004788. eCollection 2017 Oct 1.
The purpose of this study was to develop and test a nonlinear optical device to photoactivate riboflavin to produce spatially controlled collagen crosslinking and mechanical stiffening within the cornea. A nonlinear optical device using a variable numerical aperture objective was built and coupled to a Chameleon femtosecond laser. Ex vivo rabbit eyes were then saturated with riboflavin and scanned with various scanning parameters over a 4 mm area in the central cornea. Effectiveness of NLO CXL was assessed by evaluating corneal collagen auto fluorescence (CAF). To determine mechanical stiffening effects, corneas were removed from the eye and subjected to indentation testing using a 1 mm diameter probe and force transducer. NLO CXL was also compared to standard UVA CXL. The NLO CXL delivery device was able to induce a significant increase in corneal stiffness, comparable to the increase produced by standard UVA CXL.
本研究的目的是开发并测试一种非线性光学装置，以光激活核黄素，从而在角膜内产生空间可控的胶原交联和机械强化。构建了一种使用可变数值孔径物镜的非线性光学装置，并将其与变色龙飞秒激光器耦合。然后，将离体兔眼用核黄素饱和，并在中央角膜的4mm区域内以各种扫描参数进行扫描。通过评估角膜胶原自发荧光（CAF）来评估非线性光学交联（NLO CXL）的有效性。为了确定机械强化效果，将角膜从眼中取出，使用直径1mm的探针和力传感器进行压痕测试。还将NLO CXL与标准UVA CXL进行了比较。NLO CXL输送装置能够显著提高角膜硬度，与标准UVA CXL产生的硬度增加相当。
