通过放线菌酮追踪试验分析蛋白质稳定性
Analysis of Protein Stability by the Cycloheximide Chase Assay.
作者信息
Kao Shih-Han, Wang Wen-Lung, Chen Chi-Yuan, Chang Yih-Leong, Wu Yi-Ying, Wang Yi-Ting, Wang Shu-Ping, Nesvizhskii Alexey I, Chen Yu-Ju, Hong Tse-Ming, Yang Pan-Chyr
机构信息
Research Center for Tumor Medical Science, China Medical University, Taichung, Taiwan.
Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
出版信息
Bio Protoc. 2015 Jan 5;5(1). doi: 10.21769/BioProtoc.1374.
Abstract
Comparison of protein stability in eukaryotic cells has been achieved by cycloheximide, which is an inhibitor of protein biosynthesis due to its prevention in translational elongation. It is broadly used in cell biology in terms of determining the half-life of a given protein and has gained much popularity in cancer research. Here we present a full cycloheximide chase assay in our laboratory using a lung adenocarcinoma cell line, CL1-5, as a model.
摘要
通过环己酰亚胺实现了真核细胞中蛋白质稳定性的比较，环己酰亚胺是一种蛋白质生物合成抑制剂，因为它能阻止翻译延伸。它在细胞生物学中广泛用于确定特定蛋白质的半衰期，并且在癌症研究中颇受欢迎。在这里，我们展示了我们实验室使用肺腺癌细胞系CL1-5作为模型进行的完整环己酰亚胺追踪试验。
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