Kang Yuanyuan, Wang Jianbin, Davis Richard E
Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, USA.
Bio Protoc. 2017 May 5;7(9). doi: 10.21769/BioProtoc.2262.
Preparing nuclei is necessary in a variety of experimental paradigms to study nuclear processes. In this protocol, we describe a method for rapid preparation of large number of relatively pure nuclei from embryos or tissues that are ready to be used for further experiments such as chromatin isolation and ChIP-seq, nuclear RNA analyses, or preparation of nuclear extracts (Kang , 2016; Wang , 2016).
在各种实验范式中，制备细胞核对于研究核过程是必要的。在本方案中，我们描述了一种从胚胎或组织中快速制备大量相对纯净细胞核的方法，这些细胞核可用于进一步的实验，如染色质分离和ChIP-seq、核RNA分析或核提取物制备（Kang，2016；Wang，2016）。
