Eidne K A, McNiven A I, Taylor P L, Plant S, House C R, Lincoln D W, Yoshida S
MRC Reproductive Biology Unit, Centre for Reproductive Biology, Edinburgh, United Kingdom.
J Mol Endocrinol. 1988 Nov;1(3):R9-12. doi: 10.1677/jme.0.001r009.
Expression of receptors for the hypothalamic regulatory peptide, gonadotrophin-releasing hormone (GnRH), was investigated by intracellular recording from Xenopus oocytes injected with poly(A)+ mRNA isolated from rat anterior pituitary glands. Membrane depolarizations were induced in oocytes in a dose-dependent fashion following the application of GnRH (10nM - 1 microM) or a GnRH superactive agonist, buserelin (1nM - 1 microM). The response was reversibly blocked by the addition of a GnRH antagonist (1 microM). TRH (10nM - 1 microM) had no effect on most of these oocytes. In contrast, some other oocytes which showed no responses to GnRH or to the GnRH agonist, displayed depolarizing responses to TRH (10nM - 1 microM). A relatively small number of oocytes responded to both ligands. Control oocytes did not respond to the GnRH analogues or to TRH. This successful expression of the GnRH receptor could provide a new approach to the study of the receptor, and serve as a means for the isolation and cloning of the encoding genes.
利用从大鼠垂体前叶分离的聚腺苷酸尾（poly(A)+）信使核糖核酸（mRNA）注射非洲爪蟾卵母细胞，通过细胞内记录法研究下丘脑调节肽促性腺激素释放激素（GnRH）受体的表达。在应用GnRH（10纳摩尔 - 1微摩尔）或GnRH超活性激动剂布舍瑞林（1纳摩尔 - 1微摩尔）后，卵母细胞以剂量依赖方式诱导膜去极化。加入GnRH拮抗剂（1微摩尔）可使反应可逆性阻断。促甲状腺激素释放激素（TRH，10纳摩尔 - 1微摩尔）对大多数这些卵母细胞无作用。相反，一些对GnRH或GnRH激动剂无反应的其他卵母细胞，对TRH（10纳摩尔 - 1微摩尔）呈现去极化反应。相对少数的卵母细胞对两种配体均有反应。对照卵母细胞对GnRH类似物或TRH无反应。GnRH受体的这种成功表达可为受体研究提供新方法，并作为分离和克隆编码基因的手段。
